Re: [Histonet] GMS Positive Fungal Tissue Needed

2018-05-31 Thread Leonard Ringo via Histonet
Hi Kelly We have blocks that you could have. We are currently looking for gram negative, and AFB positive controls if you have any please let us know. Please let us know if you have any of those and we can work something out. Thanks, Leonard Ringo Macneal Hospital Anatomic Pathology 323

[Histonet] GMS Positive Fungal Tissue Needed

2018-05-31 Thread Pairan, Kelly via Histonet
Good Afternoon, We are in need of some GMS positive fungus tissue for positive controls. Would anyone out there happen to have some blocks we could have? We would be willing to swap if we had something you needed. We would also cover the cost of shipping. Thanks, Kelly Kelly Pairan, HT (AS

[Histonet] FW: Ventana Optiview kit

2018-05-31 Thread Lima, Teresa via Histonet
Hello Histonetters, Is anyone having issues with increased background staining of routine antibodies like keratins, CD markers and neuroendocrine markers using Ventana's antibodies with the Optiview kit? We are down to 4 minute antigen retrieval and primary antibody incubation times and still ge

[Histonet] Ventana Optiview kit

2018-05-31 Thread Lima, Teresa via Histonet
Hello Histonetters, Is anyone having issues with increased background staining of routine antibodies like keratins, CD markers and neuroendocrine markers using Ventana's antibodies with the Optiview kit? We are down to 4 minute antigen retrieval and primary antibody incubation times and still ge

[Histonet] Her2-ish

2018-05-31 Thread Charles Riley via Histonet
Does anyone run her2 ish on the Leica Bonds. If so who do you purchase your antibody from? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.ed

[Histonet] who cut the frozen

2018-05-31 Thread Cassie P. Davis via Histonet
Allison, Here and the other three labs I worked at full-time, if the frozen arrived during "scheduled hisology hours", the histology techs cut them as long as it wouldn't accrue OT, after the techs left if the PA was still there, the PA would cut them , if the histology techs and PA left, th

Re: [Histonet] Masson's Trichrome Troubleshooting

2018-05-31 Thread Mayer,Toysha N via Histonet
Tasha, Are you using the PolySci kit k037? Did they change the formulation of the Bouins? They do make one that has no picric acid, and doesn't last long. If so, add a some saturated picric acid to the Bouins to see if that helps. Are you using the Bouins again after microwaving? If so, that