Re: [Histonet] Unstained slides

2018-09-03 Thread Hobbs, Carl via Histonet
I agree: cut only the sections needed. Saves space. Sure, you lose several sections of tissue when cutting more sections. That is acceptable because, if this "oxidation" theory is true, then the initial sections will be no good. However, careful organisation of exptl procedure before actual

Re: [Histonet] Unstained slides

2018-09-03 Thread Rene J Buesa via Histonet
On this issue of lost of antigenicity, never forget air oxygen!René On Monday, September 3, 2018 11:11 AM, "Frazier, John" wrote: Interesting that you stated that, I was at the university of Colorado this past week and was speaking with the medical director of the pathology department.

Re: [Histonet] Unstained slides

2018-09-03 Thread Frazier, John via Histonet
Interesting that you stated that, I was at the university of Colorado this past week and was speaking with the medical director of the pathology department. We actually started talking about unstained slides and their storage conditions. We actually spoke of the histonet discussions around

Re: [Histonet] Unstained slides

2018-09-03 Thread Cartun, Richard via Histonet
It appears that the presence of water, both endogenously and exogenously, plays a central role in the loss of antigenicity in stored unstained slides (see reference below). Labs that are experiencing significant loss of immunoreactivity in their unstained slides should check their tissue

[Histonet] Grossing room design

2018-09-03 Thread Julio Benavides Silván via Histonet
Hi there, we are in the process of getting a new grossing room and I was wondering if there is any document or similar where the basic design requirements are stated. Something like the ventilation, material of the walls, requirements for electricity standards, pipes. Any