[Histonet] Looking for manual and block holder for Sorval JB4 Microtome

2018-09-19 Thread Ross Langston via Histonet
Hi, All. I am looking for a block holder (or ideas for suitable substitution) and owners manual for same. I have a lot of experience on the MT1, but recently came into a couple JB4s that I would like to get running. Any advice? Thanks much! Ross Ross Langston, PhD Assistant Professor of

[Histonet] Braf protocol on Bond-III

2018-09-19 Thread Greg Dobbin via Histonet
1:100 with ER-2 for 30 mins. Clone is the V600E from Spring BioScience Looks great for uis and we are performing well on EQA surveys. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!*

[Histonet] Subject: braf (v6001e)

2018-09-19 Thread Tim Higgins via Histonet
Hey Charles, I am sure you have thought about this but your retrieval process could be too stringent if you are having over expression. Thanks, Timothy Higgins, HT(ASCP), QIHC(ASCP)cm Histology Supervisor Center for Disease Detection/LabCorp tim.higg...@cddmedical.com Message: 6 Date:

[Histonet] Cytology from formalin

2018-09-19 Thread Mike Toole via Histonet
Even though this is off label I am interested in hearing input about producing a ThinPrep pap from cells collected in formalin. I suppose spinning it down and doing a Cytolyt wash followed by transfer to Preservcyt would work. Has anyone tried this or have insight into whether the fixation and

[Histonet] braf (v6001e)

2018-09-19 Thread Charles Riley via Histonet
Does anyone use this antibody on the Leica Bond systems? I have used several different vendors to validate this antibody but the Docs say all of them are over staining or not working correctly (I've gone down to 1:5000 on the dilution on all of them). If anyone has any suggestions on protocols

Re: [Histonet] histology lab temperature and humidity

2018-09-19 Thread Cassie P. Davis via Histonet
Thanks Tim, you're an excellent resource as always. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: cda...@che-east.org From: Morken, Timothy Sent: Tuesday, September 18, 2018 2:02 PM To: Cassie P. Davis Cc: