Hello, I was wondering if anyone in the histology laboratory (IHC) has a limit
on how many times one would repeat a stain; for example but not
Limited too: The tissue fell off, negative control, wrong block,etc.. for IHC
DAB and/or ISH. Thanks Ginny
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I have always "fixed" in RT acetone for 10 mins
Have compared 0-60 mins/4C to RT acetone
Lower temps just limit the rate of reaction, imho.
I note "nuclear streaming" when I use acetone at any temp/time.
Imho, acetone is not an effective fixativeit's a delipidiser.
So, give it 10 mins at RT.
Does anyone know if it is possible to work overseas? (esp. Spain)
What is the drill?
Terrence D. Wellen HT (ASCP)
Technical Specialist
LCL Pathology/IHC
Portland, OR
503-944-7923
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