good advise Carl and you asked some of the things I was wondering. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com
________________________________ From: Hobbs, Carl <carl.ho...@kcl.ac.uk> Sent: Saturday, February 2, 2019 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re guinea pig IHC Ms Ruegg, as usual , gives excellent advice: avoid HRP. Use Alk phos? Block end. alk phos by using levamisole. However, Ms Shivers does not state the fixation status of her FS. Is the Gpig tissue perfused-fixed then frozen or....frozen as unfixed...then fixed? Also....why 0.3% H2O2? Use 3%....kill the enzyme, not feed it? NOT aq for FS Make up in IMS ( 74OP) No tissue disruption However: you state that you get loadsa bubbles...so what? Is your section still attached to your slide? Can you then carry out successful IF/IHC? If yes....no problem. Sure, there's the argument that using a coagulant ( alcohol) in block is a No No I never had a problem....provided that the Formalin fixation was sufficient for unfixed crosections ( 15 mins) I do STILL severely dislike FS ( sure, I spent many years in Diagnostic Histopath doing Operative FS) Why not use Pwax sections, Ms Shivers? Curious-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 From: histonet-requ...@lists.utsouthwestern.edu <histonet-requ...@lists.utsouthwestern.edu> Sent: 02 February 2019 18:00 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 183, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C1546c2da69de40ae400f08d689398cc4%7C8370cf1416f34c16b83c724071654356%7C0&sdata=gEf%2FzAdfyRdZG0CI%2Bty4tXrOxlk5NwyD4O9qxP8DZ0E%3D&reserved=0 or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: cytology listserv (Webster, Thomas S.) 2. Re: guinea pig IHC (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Fri, 1 Feb 2019 18:28:30 +0000 From: "Webster, Thomas S." <twebs...@crh.org> To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] cytology listserv Message-ID: <93fc6a1cc62f41f5ad5890786ab04...@crh.org> Content-Type: text/plain; charset="us-ascii" Haven't seen any cytology listservs except the one for members of the ASC. There are some cytology facebook pages where you could get questions answered. This Histonet listserv is very informative. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 ------------------------------ Message: 2 Date: Fri, 1 Feb 2019 18:39:54 +0000 From: Patsy Ruegg <prueg...@hotmail.com> To: Jan Shivers <shive...@umn.edu>, histonet <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] guinea pig IHC Message-ID: <mwhpr1301mb1886debbfd50bef547312962d8...@mwhpr1301mb1886.namprd13.prod.outlook.com> Content-Type: text/plain; charset="iso-8859-1" Especially a very blood tissue like GP spleen. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com ________________________________ From: Patsy Ruegg <prueg...@hotmail.com> Sent: Thursday, January 31, 2019 11:51 AM To: Jan Shivers; histonet Subject: Re: [Histonet] guinea pig IHC In my experience it is not that GP have a higher peroxidase level, it is frozen sections in general that cannot be blocked with h202, unless they are fixed for a long time in formalin. What are others experiences with h202 blocking on frozen sections. I always used an IHC detection system that did not require h202 blocking for frozen sections. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com ________________________________ From: Jan Shivers <shive...@umn.edu> Sent: Tuesday, January 29, 2019 12:58 PM To: histonet Subject: [Histonet] guinea pig IHC Has anyone ever performed IHC on frozen sections of guinea pig tissue? I am experiencing an enormous amount of bubbling when doing the peroxidase blocking step, even though I'm only using a 0.3% concentration of H2O2. And when I say 'enormous', I mean it's like continuous champagne bubbles rising out of the tissue, even after 20 minutes in the H2O2 solution. I can't find anything in the literature that mentions guinea pigs having a higher peroxidase content in their tissues. Thanks for any help that anyone can provide. Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C1546c2da69de40ae400f08d689398cc4%7C8370cf1416f34c16b83c724071654356%7C0&sdata=gEf%2FzAdfyRdZG0CI%2Bty4tXrOxlk5NwyD4O9qxP8DZ0E%3D&reserved=0 ------------------------------ End of Histonet Digest, Vol 183, Issue 2 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet