Re: [Histonet] Processing Schedule- ASP-6025

2019-05-07 Thread Tony Henwood (SCHN) via Histonet
Processing seems adequate. After processing, how long do they sit in the embedding centre block holding tank before embedding? We found that quite a few antigens were affected when we stored control tonsil in the embedding centre (dry) at 60oC for a few days before embedding. In summary:

[Histonet] Benchmarks for Reprocessing

2019-05-07 Thread Cristi Rigazio via Histonet
Good afternoon Fellow histonetters! I was wondering if there are any tertiary institutions out there that have set a benchmark for reprocessing tissue? We tend to be less than 1%, but would love to see what others think is reasonable. Thanks for the feedback and hope you all are having an

[Histonet] Processing Schedule- ASP-6025

2019-05-07 Thread Greg Dobbin via Histonet
Hello colleagues, I recently stained (IHC) a section of normal tonsil from another facility with p16 and the resulting stain was better than the same stain on a section of my labs own normal tonsil control. This has led us to question our processing schedule. I am not concerned with our fixation

Re: [Histonet] time for penetration of methacarn fixative

2019-05-07 Thread Betsy Molinari via Histonet
John, You are such a wealth of information. I look forward to your posts. I learn something every time. That is going to be my reading list as well. Thank you. Betsy Molinari HT (ASCP) Texas Heart Institute 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari