Re: [Histonet] Processing Schedule- ASP-6025

2019-05-07 Thread Greg Dobbin via Histonet
Fascinating Tony! We don’t typically leave them in the warming drawer any longer than a couple of hours, but maybe this particular tonsil was left linger for some reason and no one thought anything of it?! Something to consider for sure! Thanks. Greg On Tue, May 7, 2019 at 9:37 PM Tony Henwood (SC

Re: [Histonet] Processing Schedule- ASP-6025

2019-05-07 Thread Tony Henwood (SCHN) via Histonet
Processing seems adequate. After processing, how long do they sit in the embedding centre block holding tank before embedding? We found that quite a few antigens were affected when we stored control tonsil in the embedding centre (dry) at 60oC for a few days before embedding. In summary: Anti

[Histonet] Benchmarks for Reprocessing

2019-05-07 Thread Cristi Rigazio via Histonet
Good afternoon Fellow histonetters! I was wondering if there are any tertiary institutions out there that have set a benchmark for reprocessing tissue? We tend to be less than 1%, but would love to see what others think is reasonable. Thanks for the feedback and hope you all are having an excell

[Histonet] Processing Schedule- ASP-6025

2019-05-07 Thread Greg Dobbin via Histonet
Hello colleagues, I recently stained (IHC) a section of normal tonsil from another facility with p16 and the resulting stain was better than the same stain on a section of my labs own normal tonsil control. This has led us to question our processing schedule. I am not concerned with our fixation

Re: [Histonet] time for penetration of methacarn fixative

2019-05-07 Thread Betsy Molinari via Histonet
John, You are such a wealth of information. I look forward to your posts. I learn something every time. That is going to be my reading list as well. Thank you. Betsy Molinari HT (ASCP) Texas Heart Institute 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari