I've never seen the kind of staining you describe, abi jag, with "the complete
section become stained as red" but I've never used the method on sections of
liver.
You should get red collagen and yellow hepatocytes with blue nuclei. The
strongly acidic picrosirius stain, applied for an hour,
Subject: PAS on the ST5020
Hello,
Does anyone run PAS on an ST5020? If so, would you mind sharing the
protocol that you use? I am asking on behalf of one of my customers that
needs to program a PAS protocol on her ST5020.
Thank you very much!
Best,
*Justin Rabidoux*
www.rankinbiomed.com
Hello Histonetters,I am writing this to seek your help regarding a very recent
problem that I am currently facing with Picro Sirius red staining of lab animal
(mouse and rat) liver samples. I follow the procedure that was provided by John
Kiernan in the histonet archives (please see below),