Re: [Histonet] Recent issues with picro sirius red staining (entire liver section become red, no yellow background)

2019-08-09 Thread John Kiernan via Histonet
I've never seen the kind of staining you describe, abi jag, with "the complete section become stained as red" but I've never used the method on sections of liver. You should get red collagen and yellow hepatocytes with blue nuclei. The strongly acidic picrosirius stain, applied for an hour,

[Histonet] PAS on the ST5020

2019-08-09 Thread Rob Rankin via Histonet
Subject: PAS on the ST5020 Hello, Does anyone run PAS on an ST5020? If so, would you mind sharing the protocol that you use? I am asking on behalf of one of my customers that needs to program a PAS protocol on her ST5020. Thank you very much! Best, *Justin Rabidoux* www.rankinbiomed.com

[Histonet] Recent issues with picro sirius red staining (entire liver section become red, no yellow background)

2019-08-09 Thread abi jag via Histonet
Hello Histonetters,I am writing this to seek your help regarding a very recent problem that I am currently facing with Picro Sirius red staining of lab animal (mouse and rat) liver samples. I follow the procedure that was provided by John Kiernan in the histonet archives (please see below),