Terri, when I was in animal research we used the blood from the animal to make plasma and combined it with some thrombin from the hospital pharmacy. Of course that is tedious especially since histogel is available. I warmed the histogel by placing a tube of it in a beaker of hot water, do not mw it.
Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com ________________________________ From: John Garratt <john.garr...@ciqc.ca> Sent: Thursday, January 23, 2020 10:51 AM To: Terri Braud <tbr...@holyredeemer.com> Cc: histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Need a procedure Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good for IHC and does not have the pitfalls of plasma/thrombin. Plasma/thrombin does work well for cell blocks but you will have to consider an ethical and safe source for your plasma. The instructions for using Histogel are in the package insert though I have one comment. Be careful how you warm the Histogel and use a heat block. Do NOT use a microwave since there is a tendency to overheat the gel and you will end up with poor quality IHC. John www.cpqa.ca<http://www.cpqa.ca> ‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐ On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet <histonet@lists.utsouthwestern.edu> wrote: > Hi fellow Histonetters - I'm in need of some help, please > Background - We currently use agar to capture our scant cell blocks for > processing. I am unfamiliar with the Plasma/Thrombin method of cell block > preparation and am interested in comparing it to our current method > Request - Could you please send me your procedures for this method, > specifically where you purchase your plasma and thrombin and what species are > used? > Thanks in advance. Histotechs rock! > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
