Re: [Histonet] Spectra

2020-04-21 Thread Gudrun Lang via Histonet
Hi! Do you use your own reagens or the Leica Spectra dyes? We have house-made ones. Too faint eosin staining can be rendered more intense with a few drops of acetic acid into the eosin to reach a pH about 5. Second, it is often a prolonged washing after the eosin that causes excess stain to be

Re: [Histonet] Formalin fixation for COVID-19 positive tissues .....

2020-04-21 Thread E. Wayne Johnson via Histonet
Where aldehydes are used for disinfection we consider 30 minutes contact time for difficult viruses like African Swine Fever (ASF) virus to be adequate.  Coronaviruses like PEDv TGE IBV and the SARS virus are much easier to inactivate than ASF virus. I'd consider the SARS virus to be similar to