Re: [Histonet] Histology text book "bibles"

2020-07-09 Thread Muhammad Azam via Histonet
These last few posts r so wonderful and revealing. Where we came from and all the glorious history. Very nostalgic Dr Azam Sent from my iPhone > On Jul 9, 2020, at 2:44 PM, Hobbs, Carl via Histonet > wrote: > > Good to read of others' xp > I remember the Armed Forces book...good in parts,

[Histonet] Histology text book "bibles"

2020-07-09 Thread Hobbs, Carl via Histonet
Good to read of others' xp I remember the Armed Forces book...good in parts, unlike the Curate's egg! Disbrey and Rack...wonderfully refreshing. Exceedingly well-informed person was Brenda! I reckon she swung the panel in my favour for a Histology Manager job.YEARS ago! Imho the forerunner

Re: [Histonet] Crystals forming in Hematoxylin

2020-07-09 Thread Paula Keene Pierce via Histonet
That is the aluminum salt precipitating out. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, July 9,

Re: [Histonet] Crystals forming in Hematoxylin

2020-07-09 Thread Hannen, Valerie via Histonet
Hi Terri, The crystals form during cold winter months, usually during shipping or can form if the Hematoxylin 7211 is stored in a cold environment. They also form due to the Mordant being put into solution at it's saturation point. The crystals have no adverse effect on the stain itself. If

[Histonet] Crystals forming in Hematoxylin

2020-07-09 Thread Terri Braud via Histonet
Hello Histo Peeps - What are the clear crystals that form in Hematoxylin? We use Richard-Allen 7211. We love the stain, but occasionally, when a bottle has been previously opened, we find clear crystalline precipitate forming in the bottom. Today, we had a huge one (over an 2" in greatest

[Histonet] Frozen tissue

2020-07-09 Thread Anne Murvosh via Histonet
I'm testing a faster Mart 1 for melanoma on Mohs specimens. Its been a while since I've done any frozen tissue IHC stains and I can't remember the fixation to keep it viable for testing the next day, or same day but later on. Will it hold up if fixed in acetone and dried or do I need to