Hi Karen,
The best resource for Cyto questions would probably be a recent edition
of the Carson Histotechnology book. The Fourth edition has some info on
special stains for Cyto, but the new Fifth edition is expanded on that
topic and has more info on IHCs for Cyto (Chapter 17).
For
Ms. Heckford:
As a cytotechnologist with just a little more than average experience with
immunostaining procedures, I’d like to offer my input. To my knowledge, there
are very few publications that address your concerns. I have, however,
collected/prepared a significant amount of
Hello Histonet!
Can anyone share their protocol for cutting frozen section (rolls) that will be
used for RNA sequencing downstream much like this nature article:
https://www.nature.com/articles/s41591-020-0844-1
The only direction we have been given is to cut the sections at 100 microns.
Steve, I agree with your whole thing. And our pathologist fought against
plastic for years simply due to the fact it scratches easily and we are
constantly pulling and filing slides for various needs. However, when we
decided to go to scanning slides wet mounting media was never going to work -
I respect your view point but as someone who has trained histotechs and
cytotechs, the film leaves residual dots from dotting pens making it a
challenge to test individual's ability to locate and identify tissues and cells
of interest. That is the only draw back that I have seen in my years
Very interesting paper John! Thank you. I wish the authors had also
experimented with higher concentrations of formaldehyde (eg 10% formalin).
Might one infer that 10% would be even more efficient in inactivating viral
infectivity than 2 and 4%? 樂
Cheers
Greg
On Thu, Aug 6, 2020 at 11:02 AM John
Evaluation of Virus Inactivation by Formaldehyde to Enhance Biosafety of
Diagnostic Electron Microscopy
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353909/
It is nice to have a reference.
John
On Thu, Aug 6, 2020 at 4:10 AM, Greg Dobbin via Histonet
wrote:
> Hi Amy,
> Formalin fixed
Hi Amy,
Formalin fixed tissue is no longer infectious...unless you are talking
about prions (eg scrapie, BSE, etc). So there should otherwise be no
concerns or additional precautions required.
Cheers,
Greg
--
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE C0A 1P0
*Everything in
Hi Jeanine,
- Film coverslips scratch very easily. Any dust in the system will scratch the
film.
- Staining is often compromised if the film coverslip needs to be removed.
- Specific storage conditions are required to validate the adhesive on the
film. If the storage conditions are not met the
