This is all good advise. I always picked up section and tapped the slide on counter and then dried standing up. This is really a problem if you are at all above 5K feet in altitude where water boils violently at 92-93dc, which will destroy your tissue section. I have written a couple of papers about on this.
________________________________ From: Greg Dobbin <greg.dob...@gmail.com> Sent: Friday, January 22, 2021 11:45 AM To: b-freder...@northwestern.edu <b-freder...@northwestern.edu> Cc: histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Water under sections Hi Bernice, In my lab, water under the sections is unique to charged slides. And you are correct, if there is water under the section when the slides are heated for antigen retrieval, the boiling (or at least very hot) water will damage or entirely destroy the section. We allow the charged slides to drain (upright) for a few minutes and then carefully make a hole in the edge of the wax and use a Kimwipe or paper towel to carefully wick the excess water out as much as we can without touching the tissue section. Then we "flick" or shake sharply to remove any residual water that may remain before we bake them (FYI, we choose not to bake on the stainer). If after baking 30 mins at 60C, we notice water (maybe someone was not as diligent earlier?), we wick away the excess and bake for another 15 mins to ensure good adhesion of the section to the slide before proceeding to the immunostainer. Hope this helps. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
