Use DAPI/HOECHST at the same concentration you use for cells/FS
I use Hoechst H33258 Sigma 10mg/ml soln
I dilute by adding 0.5 microL to 100 microL buffer then add 1/100 to Alexa
secondaries ( final 1/20K diln factor)
Some incubate in HOECHST or DAPI after secondary ab incubation, for IF
Sure, if
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Dear Histonet list,
I have made some paraffin blocks of demineralized bone and cartilage. I
would like to stain paraffin slides with DAPI (or another DNA stain).
Does DAPI staining (or other fluorescent DNA stain) work well on paraffin
sections? Does anyone have a protocol they would like to
