Re: [Histonet] Unstained slides precut for IHC

2021-06-13 Thread Tony Henwood (SCHN) via Histonet
Hi Carrie, Over-heating of sections (and tissue blocks) for IPX is probably one of the most significant and unappreciated pre-analytical factors that can affect immunolocalisation. We closely scrutinise slides closely for overheating, especially those sent to us for immunostaining. We strongly

[Histonet] Unstained slides precut for IHC

2021-06-13 Thread Carrie Disbrow via Histonet
Hi! Current lab is cutting extra slides for IHC and putting in 60 degree oven for 45 mins to dry. If IHC is ordered a precut control and the already baked slides are again put in the 60 degree oven for 45 mins. Sometimes the control is cut and added to the dry slide or a separate slide. So,