Make sure the periodic acid is made fresh EACH time you run the stain.
That can also make a big difference in the stain quality.
Colleen Forster HT(ASCP)QIHC
On Thu, Sep 23, 2021 at 6:14 PM Tony Henwood (SCHN) via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> I agree with Bryan,
>
>
I agree with Bryan,
The introduction of thiosemicarbazide before the silver step improves the
staining immensely.
I would also look at the periodic acid. Is it too dilute, though 0.5% should
work? I usually cover this by using a 1% solution for 20 minutes.
Regards
Tony Henwood JP, MSc,
Hi,
Try the method given in StainsFile at:
http://stainsfile.info/stain/metallic/jones.htm
Bryan Llewellyn
Hood, Jordan via Histonet wrote:
Hello,
I'm new to histology (and new to histonet), and I work in a small histology lab
specializing in animal tissues that receives
Martha,
We reported our study of 15 brands of adhesive slides for "wash off" and
found little difference among the different slides when well-fixed cell
culture material was examined.
On the other hand, poorly-fixed breast cancer tissues did appear to adhere
more strongly to some slides than
It was brought to my attention that we had significant washing on 3 of 8 bone
marrow clot sections the other day; this is not the first time so we would like
to get to the bottom of this. We use positively charged slides and all 8
cases were cut and run the same morning but allowed to air
Hello,
I'm new to histology (and new to histonet), and I work in a small histology lab
specializing in animal tissues that receives requests/submissions from
researchers. I tried (and failed) to perform a Jones' Methenamine Silver stain
on a client's submission of pig kidneys (formalin-fixed,
Hi Leslie,
I have attached our current log. You can augment it based off the solutions you
are using and how many shifts you have. We have two stainers and each has their
own log. Our stainers are connected to running water thus the drain lines that
need cleaning. If you have any questions
