Re: [Histonet] Tissue staining artefacts - areas of tissue not labelling

2022-06-15 Thread Greg Dobbin via Histonet
Hi Peter, We need more info: 1. What is the tissue(s)? Species? 2. What is the marker(s) in question? 3. Answer 1 and 2 with regard to the shared images? 4. What is the expected staining reaction? 5. Is there a control section placed on the same slide? If yes... 6. How does the

[Histonet] Tissue staining artefacts - areas of tissue not labelling

2022-06-15 Thread Peter March via Histonet
Hi, A lab which does a lot of immuno work on tissue sections (both DAB and fluorescent labelling) have run into issues with uniformity of antibody staining. Some areas of the tissue just don’t label and it seems to be a very clear line between the stained and unstained tissue. The tissue was