Your technique is the one first (I think) published by Lopez-De Leon A &
Rojkind M (1985) A simple micromethod for collagen and total protein
determination in formalin-fixed paraffin-embedded sections. J. Histochem.
Cytochem. 33: 737-743. The photos in that paper show some of the collagen
We are working out an alternative method for collagen staining using Fast Green
/ Sirius Red (Direct Red 80) rather than the standard picrosirius red method as
I think it is prettier and easier to see the collagen on a green background.
What we’ve noticed, though, is that we are seeing
