Re: [Histonet] Von Kossa staining

2023-07-26 Thread John Kiernan via Histonet
Charles, A handheld light of any kind isn't really suitable because you would have to hold it over the slides for 15 to 60 minutes, according to which variant of the von Kossa method you plan to use (see Lillie & Fullmer 1976 Histopathologic Technic ... 4th ed. pp 539-541). An anglepoise

Re: [Histonet] Von Kossa staining

2023-07-26 Thread Debra Siena via Histonet
Hi Charles, I can't answer your question about UV light as I've never tried it except with incandescent lights. However, I do know that you could use natural sunlight as well to develop the silver color change. I hope that helps, Thanks Debbie Siena, HT(ASCP)QIHC Director of Scientific

[Histonet] Von Kossa staining

2023-07-26 Thread Charles Riley via Histonet
Can anyone out there who performs Von Kossa staining provide me with any guidelines or suggestions for the light source to use for the Silver nitrate activation? Is a standard handheld black light strong enough or does it need to be a UV sanitizing strength light if using UV versus incandescent