The Sudan black B method for lipofuscin is exactly the same as for frozen
sections, but it is applied to hydrated paraffin sections. Remember that the
dye solution needs to be fairly fresh (less than 4 weeks old) and must be
filtered immediately before using. Use Sudan black B (CI 26150) powder
Hi,
I have been asked to do a Sudan stain on a heart biopsy for lipofuscin. The
biopsy is in a paraffin block. They are looking to better report and
understand the IHC. I am totally unfamiliar with this stain. I did some reading
but have been unable to find a protocol for paraffin sections. I
