We embed from cold and this happens if the tissue is not brought up to the
temperature and the wax is melted.
-Original Message-
From: Carl Hobbs via Histonet
Sent: Friday, February 9, 2024 11:12 AM
To: histonet
Subject: Re: [Histonet] tissue cassettes
EXTERNAL SENDER - Exercise
I'm interested but don't understand the variation and it's + effect
I take my cassettes out of the processor and immediately place into the molten
wax bath of the embedder ( if I'm embedding immediately; if not I let the
cassettes/tissues therein go cold until a later embedding)
When embedding
>
> Re: [Histonet] Modified Davidson
>
> The formula for "modified Davidson's fixative" that I used was three parts
tap water, three parts reagent alcohol, two parts 37% formaldehyde ("strong
formalin"), one part glacial acetic acid. Best mixed under a fume hood.
At 85 I must be one of the last
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I am old school and prefer them dry, lol. I agree with Thomas, that shouldn't
have that affect.
Ginny
-Original Message-
From: Thomas Podawiltz via Histonet
Sent: Friday, February 9, 2024 8:34 AM
To: Brazie, Jeneanne E *HS ;
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet]
Hi,
Since we have turned to embedding centers in the late 80ies we let the
cassettes sit in the centers without additional paraffin.
We only see such "jumping out" tissue, when the cassettes are not warmed
(let the lid open) and the tissue renders too cold. As a result tissue and
paraffin don't
Without seeing the blocks, that sounds more like cold molds being used, more
Then, whether or not the tissues are kept in a dry, hot, well, or a wet well.
Sent from Yahoo Mail for iPad
On Friday, February 9, 2024, 6:00 AM, Brazie, Jeneanne E *HS via Histonet
wrote:
Hello :) I am
Hello :) I am encountering push back in our lab when I fill the embedding units
with melted paraffin
in the embedding wells. The techs here like for the tissue cassettes to sit
dry (no wax) while in the
embedding units. I find that the tissue rolls out of the sections while
cutting because of
