[Histonet] Cryotomy help

2024-07-13 Thread Carl Hobbs via Histonet
I assume that the fresh tissue was fixed in methanol? If so, it is irreversibly coagulant- fixed ( like boiling an egg white...no going back) Place the specimen in dist water until it melts to RT x3 changes of dw 1hr each at RT on a gentle rocker to get rid of methanol ( antifreeze, hence inabi

[Histonet] NBF usage in the world

2024-07-13 Thread Gudrun Lang via Histonet
Hi! Is it correct to say, that NBF is used in nearly every histolab in the world as standard-fixative? Here in Austria, I don't know any lab, that uses any other fixative. Nor any formalin-substitute. There have to be a few labs, that work with the Sakura XPRESS and their UMfix? What's about