[Histonet] Marker for Rabbit B-cells

2009-04-08 Thread agrobe2555
Can anyone provide me with information for antibodies that will detect rabbit B-cells/plasmacytes?? This would be used for FFPE tissues. Thanks Albert Grobe, PhD Tissue Engineering Lab International Heart Institute of Montana ___ Histonet mailing list

[Histonet] Hematoxylin staining and Calcification

2008-11-25 Thread AGrobe2555
Hello all, Is there anything published on using H&E staining to presumptively indicate calcification (primarily the hematoxylin)? Thanks, Albert Albert C. Grobe, PhD Tissue Engineering Lab International Heart Institute of Montana Foundation **One site has it all. Your email acc

Re: [Histonet] RE: Is it Formalin-Without Smelling It?

2008-11-07 Thread AGrobe2555
In our lab, if it isn't labeled, it gets thrown out. But we work in research and not in a clinical setting Albert Albert C. Grobe, PhD Tissue Engineering Lab International Heart Institute of Montana Foundation **AOL Search: Your one stop for directions, recipes and all ot

[Histonet] Complement activation and Thrombin generation

2008-11-03 Thread AGrobe2555
Hello, I am looking for assays (either chromogenic or fluorescence based) for both complement activation and thrombin generation for a proposal. The ones suggested by the sponsor are not available in this country. Any suggestions/sources? Thanks, Albert **Plan your next getaway

Re:[Histonet] need help for cultured cells

2008-10-23 Thread AGrobe2555
You'll need to resuspend your pellet in a limited volume, do a cell count (using a hemocytometer, etc) and dilute the original suspension to the desired cell#/ul concentration with an appropriate diluent. Albert Albert C. Grobe, PhD Tissue Engineering Lab International Heart Institute of Mo

[Histonet] Re: Which one first - antigen retrieval or peroxidase block

2008-09-29 Thread AGrobe2555
I'm with Rene on this one. Block after retrieval. For those of you who do the block before retrieval, how do you control for any activity that might be exposed during the retrieval step? Couldn't it lead to false positives in your slides? Albert C. Grobe, PhD Tissue Engineering Lab In