[Histonet] Multiplex IF staining

Hello Hist-world, Does anyone have experience doing FISH/double IF staining? My newest project is to combine a FISH probe (texas red) with anti-Staufen IF (green secondary) and anti-MBNL1 IF (secondary TBD). So I've got a red, green, and blue (DAPI counterstain) already. If I used a yellow

[Histonet] Fixation for FISH

Hello Histo-land, I'm going to start doing FISH in our lab, but I've never done it before. I read that the PFA fixative solution should be made fresh; however, I have some PFA that is being stored in the -80 freezer (for less than a year). Does anyone know if this would be ok to use? And

[Histonet] GI Pathologist Consulting

Hello all, I was wondering if anyone knows of a GI pathologist interested in doing a quick side project as a consultant for the company I work for? If so, please message me privately and I can give you the details. Thanks so much, Adrienne Anderson

[Histonet] Decal of bone marrows

Hi Histo-land, I have a really quick question: about how long does it take to decal a bone marrow biopsy? Thanks a bunch! Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] HBV Control Block

Hello all, We are in need of a HepB control block. Does anyone have any recommendations for procuring this? I actually only need a few slides, so I’m just feeling things out. Any advice would be appreciated. Thanks! Best regards, Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist

Re: [Histonet] Eosin Leaching

, that could be causing the problem. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 19, 2014 5:26 PM To: 'Adrienne Anderson'; 'histonet

[Histonet] Eosin Leaching

in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information

[Histonet] RE: Adrienne Anderson

Hello! http://www.adicea.com/lrgo/vmc/azyir/ajxo.htm Adrienne Anderson vgzb ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Xylene/Paint Thinner

I have to admit, I don't mind the smell:) But I also like the smell of gasoline, and I know both are bad for me! Thanks again to everyone who has offered feedback on this topic. Rene, I have printed out a few of the articles you've written and am anxious to research these methods more. Thanks

Re: [Histonet] Xylene Substitutes

Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Tuesday, February 05, 2013 4:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitutes Hello all, My lab is looking

[Histonet] Xylene Substitutes

Hello all, My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical? Thanks, Adrienne ___ Histonet mailing list

[Histonet] Stainer for sale

Hello all, We have an automated stainer we are looking to sell. It's a Microm DS50. If interested, please email me. Thanks! Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] Happy Retirement to Jim Burchette

Jim is such an amazing person and an expert in his field. He will be missed in the Immuno lab, I'm sure! Congrats, Jim! Sounds like you'll be able to enjoy some more fly fishing time!! On Jun 21, 2012, at 1:50 PM, Teri Johnson wrote: I am making this official and inviting all of you who

[Histonet] Used block cabinets

be appreciated.  Thanks! Best, Adrienne Anderson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Research microtomes

the article mentioned a microtome that could cut 50-2000, we are most interested in the range around 50-150 microns. Thanks, Adrienne From: Jack Ratliff ratliffj...@hotmail.com To: Adrienne Anderson rennie1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu

Re: [Histonet] Research microtomes

Hi Jack, We're trying to cut just plain old FFPE blocks. From: Jack Ratliff ratliffj...@hotmail.com To: Adrienne Anderson rennie1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; bush...@rose-hulman.edu bush...@rose

[Histonet] Job opening in Columbus, OH

Hello Histo-land, We are a small biotech company seeking a part-time HT or HTL for our laboratory. This would be ideal for someone with a full-time job looking for additional work hours. As our work load fluctuates, you must be flexible and willing to go with the flow. Experience in

[Histonet] Need help with an old autostainer

trouble setting up a staining program), but I don't quite know if I'm going about it the right way...or if there is one I can actually get. Does anyone know anything about this type of stainer. Please help! Thanks, Adrienne Anderson Phylogeny Inc. Columbus, OH

Fw: [Histonet] Need help with an old autostainer

Thanks for all the great responses. I have found a manual and lots of advice! Thanks, Adrienne - Forwarded Message From: Adrienne Anderson rennie1...@yahoo.com To: histonet@lists.utsouthwestern.edu Sent: Tue, January 4, 2011 12:19:13 PM Subject: [Histonet] Need help with an old