[Histonet] Thionin Staining Recipe and Protocol

my own troubleshooting or even recipes and protocols would be greatly appreciated! Thanks, as always, for being such wonderful resources for those like myself who have such little histology background :) Amanda Madden ___ Histonet mailing list Histonet

[Histonet] Leica Service Technician

Hi Histonetters! A few months back I emailed regarding a service contract through Leica for our CM 3050S, and (unfortunately?) we chose not to purchase one. This week we have had a serious issue with it... the specimen head and arm is covered in frost, and the object temperature sensor is reading

Re: [Histonet] Leica Cryostat Service Recommendations

t; restart your cryostat after defrosting, cool the chamber first, then > specimen head. That way, the specimen head (object temperature) > compressor > will not have to work as hard to keep itself cool while the chamber is > cooling. > > Emily > > A great book sh

[Histonet] Leica Cryostat Service Recommendations

Hello Histonetters!!! I am writing today to ask about the necessity of having cryostats serviced regularly. We have a Leica CM 3050S that is about 2 years old now. I have been looking far and wide for recommendations about cryostat maintenance, but haven't found much information. Do you think it i

[Histonet] Nickel-DAB Signal Fading

x27;t think it is light. Going crazy, and thankful for any advice, Amanda Madden ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Losing Sections While Dehydrating

problem but I'm not sure. Thanks in advance. Amanda Madden Research Assistant P.S. The subbing protocol that I used only called for one "dip" into the gelatin solution, and didn't call for any type of acid wash. Also, our cryostat is a Leica CM3050S and I was wondering if a