For those of you who have Vantage, in your grossing room do you have Vantage
set up or your LIS system? I was thinking it should be vantage, but our
Co-path person said we should have AB&T set up in the grossing room and let
vantage start at embedding, microtomy and case assembly. What are yo
Does anyone else have problems with what looks like squamous cells staining on
your H&E's and IHC's? I'm trying to figure out how to eliminate that problem
in our lab...wear gloves while cutting? Change out water bath several times
during shifts? Any suggestions? Thanks!
___
BUT, there is no answer key! I don't like that. I wish they were in the back
of the book to review.
Amber McKenzie | Histology Supervisor
GI Associates & Endoscopy Center
1405 North State Street, Suite 400
Jackson, MS 39202
Phone | 601-863-0381
Fax | 601-326-3532
-Origina
For those of you who have a Symphony, how do you wick your slides? Do you cut
them and put them in a basket to drain and then transfer to the trays? Do you
cut them and lean them up against your water bath? Do you out them in the oven
for a few minutes before you load them onto the symphony?
For those of you who have the Symphony, how long do you let your slides dry
before filing them? How do you keep each day separate and how much
room/organization do I need? We just got one and filed our slides after a day
and now they are stuck together like a brick! Just trying to figure out t
I have a new piece of equipment being installed in my lab, does anyone have a
generic competency form you'd like to share? I need something for our staff to
sign saying they were trained on it. Thanks!
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Does anyone know of a specific site and quote that states "No food or drink in
the lab" and what the consequences would be? That covers drinks/snacks in the
cabinets above their workstations too, right? I have a sign up on the door
entering the lab that states no food or drink, but the staff co
I ordered the MSH online study guide for the QIHC, but it was only
questions...no answer key in the back. What books am I supposed to get the
answers from? Any other study suggestions? Thanks!
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When staining PAS's, does the Schiff need to be room temp or does it matter?
Just curious about when to add it to our Sakura stainer. Should we put the
schiff's on at the beginning of our shift so it's room temp when we need it or
when should add it when we add PAS slides to it that need to b
Can you forward any templates to me as well? Thanks!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leslie Graves
Sent: Tuesday, July 15, 2014 8:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histo
What should the water volume be? Temperature and clarity? How do you test
these things?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Shelley
Sent: Wednesday, July 09, 2014 12:39 PM
To: Nancy Schmi
For those of you who have Vantage, which block printer do you use? I've heard
Leica has one with 3-6 hoppers that can print the 2D barcode on it pretty fast.
I currently have the TBS block printer, but I'm told it can print the bar
code, but very slow...Any suggestions?
Thanks!
_
Does anyone use the Thermo High-Capacity Section Dryer Item number B3120202?
I've never seen it in person and I was debating on getting it vs the Cardinal
one: M7289-20 SD-II-120 Slide dryer II. Any advice? Thanks!
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Does anyone have any recommendations on a small slide oven to sit by the
microtome to put immunos/specials in before staining? I have one I bought from
Cardinal maybe 2 years ago and it's acting up. And the thermometer that sticks
out the top is acting crazy as well.
Thanks!
_
Does anyone know where I can find a list of all the special stains/IHC stains
with their appropriate CPT code? Is there a book to buy or can I google it and
print something off? Thanks!
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htt
Can you pass it on to me as well? I'd love to compare what I've got to what
someone else is doing.
Thanks,
Amber
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald
Sent: Tuesday, April 08, 2
Would it be rude to ask other hospitals that review my slides to NOT WRITE
their accession number on the front of my slides, but instead put their
accession number on the back of my slides? Sometimes, I get slides back that
have stickers on the front under my accession number or they'll hand wr
The Mississippi Society for Histotechnology meeting will be held in
Hattiesburg, MS on Saturday, June 28th, 2014. All is welcome! If you have any
questions, please feel free to contact me.
Thanks!
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Are there any regulations specifying any difference of holding blocks/slides
for peds cases? Are they treated the same as adults cases and held 10-12 years
or are they needed to be kept longer?
In the block/slide storage room, are there any regulations on temp or humidity?
Thanks!
_
2 questions:
1. How do you document who grosses in your specimens? Are their initials typed
on the report under gross description or are they hand written on the paperwork
that comes with the bx's - path requisition?
2. How do you document that the special stain/IHC (+) and (-) slides
worked/fa
So, Ventana came out to my lab to do a LEAN consult for us since we are
building a new lab...they want to sell us their vantage tracking system and H&E
stainer. Our Rep is coming on Fri to talk to me, our lab director and CEO
about these products. Anyone using these? I'd like some feedback s
Has anyone had experience with Ventana's Lean team? I am correctly designing a
new lab and they are coming out next tues to look at my workflow. Just
wondering what to expect and how much they may change my current layout of my
future lab :)
Thanks!
_
Do you any of you have your accessioning area right next to your grossing
station so that the person accessioning can put cases on a cart and the person
grossing can just grab each case and gross it in as they are being accessioned?
Just curious about this setup. Thanks!
Where do you get your spill kit? Do you have a company and part number? Thanks!
-Original Message-
From: Vincent Rivera [mailto:vriv...@westderm.com]
Sent: Tuesday, January 07, 2014 11:31 AM
To: 'Rathborne, Toni'; 'Eytalis, Robert A'; Anne Murvosh; Amber
What safety items do you keep in your lab besides the normal apron, gloves, eye
wash stations and shower? Thanks!
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I'm currently designing a new GI lab. Any advice on grossing stations? The
only name I know is Mopec. Is that the best one to go with or are there better
ones out there? Thanks!
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http://lis
We haven't started this procedure yet. I'm just trying to get an idea of what
to expect and what to order to be prepared.
-Original Message-
From: Joe W. Walker, Jr. [mailto:joewal...@rrmc.org]
Sent: Thursday, November 21, 2013 3:38 PM
To: Boyd, Debbie M; Amber McKenzie
Cc
Do any of you do Esophageal Brushings in your lab? If so, what do you use to
stain the slides...just coplin jars side by side dipping the slides in or some
type of stainer? Who do you order your supplies from? There were a couple of
stains I didn't recognize in the protocol we are supposed to
What's the height limit you can stack blocks/slides according to CAP?
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We are looking at buying new chairs for our lab...any suggestions?
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I am currently redesigning our GI lab. I wanted everyone's opinion on if you
could redesign your lab, what would you change/add/delete? Would you do
counters high or low? What flooring would you use? What brand/type of
countertop would you use? I'm trying to design it for lots of extra space
Does anyone know where I can find a wall mount that you put your clean dishes
on to dry? We currently just lay a paper towel on the cabinet to let our
dishes dry on, but I'd like to find a the wall mount that has areas where you
can hang the items up to dry. Thanks!
_
On the daily embedding log, do you have your embdedders initial each case they
embed or do you allow them to initial the first case on the page and then draw
a line down the page symbolizing they did all of those? How detailed should it
be? And, you keep these for 2 years like all other paper
Do you guys keep the QA form when a specimen is rejected and sent back to the
source for correction? ANP. 11475
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I'm trying to get our Co-path computer system set up to print labels instead of
us handwriting on the paperwork and containers...what information do you guys
have on your printed labels...just patient name and accession no? Thanks!
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When accessioning, do you label the paperwork AND the container with the
surgical number? If so, do you hand write the number or use a label maker?
Also, do you keep your embedding logs for 2 years like CAP requires QA and QC
records?
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I'm cleaning house :) Anyone interested in buying these old books?
1. A color Atlas of Histology by Dennis Strete, ISBN 0-06-501084-1
2. Human Histology, 2nd edition, by Alan Stevens and James Lowe, ISBN
0-7234-2485-3
3. Immunolgy, 4th edition, by Roitt, Brostoff, Male ISBN 0-7234-2178-1
4. Atl
I used to use Amphyl to decon my Ventana equipment, but now Cardinal sells
Lysol IC in its place and I was wondering how you guys make it up...water to
cleaner ratio in the Vantana carboys. With Amphyl I did 20L dH20/200ml Amphyl.
Thanks!
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What do you guys use to clean your Gross Room utensils? I bought Lem'in Lift
from Mantek in the past to soak the rulers, scissors, tweezers, etc...Thanks!
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Does anyone have a checklist for EPA? I had 2 representatives stop by
unannounced and cite us for several things. Thanks!
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Does anyone have a checklist for EPA? I had 2 representatives stop by
unannounced and cite us for several things. Thanks!
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Does anyone have a flow cytometry protocol they'd like to share?
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I have 2 employees that are wondering about seniority... one HT has been on
payroll for 5 years and has gone from FT to PRN and back to FT without any
breaks. The other tech has been FT for 3 years. Which one is the senior in the
lab?
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Has anyone validated the new Ventana Benchmark Special Stainer? I was
wondering what the standard slides count was? I would figure less than
validating the immuno instruments, but wanted to check with everyone.
Thanks!
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Does anyone have a QC log on the yearly fire shower testing they'd like to
share?
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For EPA standards, how do you label your waste drums? I have one 55 lb drum
that we only pour ventana waste in. What numbers do you write in the diamond
to cover all the kits that you stain with? 2 reps from EPA came by my lab
yesterday and said i needed an emergency phone list by our phone??
Does anyone know where to find the High complexity testing requirements for
CAP? We're about to apply for CAP accreditation and want to make sure the
person working in our lab grossing has the requirements. Thanks!
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Just curious what everyone else does...I've decided to do the even's 1 quarter
and the odds the next quarter (because of cost)...do you just run them,
document it and throw them away? Or does anyone make a copy of them and save
in the QC book? Thanks!
_
I was wondering what career ladders where in your labs? What goals do
employees have to meet before they get a pay raise/advancement in the lab/ or a
new title?
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How do you not cut thru the bead when sectioning? I'm intrigued by this
process b/c I've never heard of this before.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd
Sent: Tuesday, June 26, 201
Does this slow the embedder down any having to pick a bead up each time to
embed along with the tissue? I've never heard of this procedure before. The
labs I've worked in have an embedding log and we initial each case we embed.
What do you keep the beads in and are they set in a particular spo
How many hours of Science does a person need to be qualified to Gross (under
CAP and CLIA)?
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Okay, let's move on people. It's getting too personal instead of professional.
Enough already
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline
Sent: Tuesday, April 10, 2012 12:19 PM
To: Ni
Are there any guidelines on how to do GI grossing? At what size to you bisect?
Do you call 5 or more pieces multiple or do you count all the pieces? How
many categories are there: 1, 2, 3, several, multiple, etc...At what size is it
considered a fragment and what size is a polyp? Thanks!
_
s and proficiency testing?
-Original Message-
From: Rathborne, Toni [mailto:trathbo...@somerset-healthcare.com]
Sent: Tuesday, March 27, 2012 3:27 PM
To: Amber McKenzie; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] CLIA requirements
We keep all of ours in the lab. Much easier
-boun...@lists.utsouthwestern.edu wrote: -
To: Amber McKenzie
From: Victoria Baker
Sent by: histonet-boun...@lists.utsouthwestern.edu
Date: 03/27/2012 10:13AM
Cc: "histonet@lists.utsouthwestern.edu"
Subject: Re: [Histonet] CLIA requirements
Hi Amber,
Try contacting HR and the Compliance Office
CLIA requires me to keep a personnel file on everyone that works in the lab and
I was wondering how you approach the staff about turning in their highest level
of education. Do they bring in a copy of their HS diploma? College diploma?
And what if they're not finished with college, do they o
Is there a difference in using DI water vs tap water in the water bath?
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Do y'all run the temp verifier slides for the quarterly maintenance for the
Ventana XT and Ultra? Those slides are mighty expensive to buy every 3 months,
esp if you have multiple pieces of equipment.
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What % QA do most pathologists do internally? I'm trying to update my AP
Manual. My last CLIA inspection, they told me to put in my AP Manual that it
is done and get a binder labeled "discrepancies". If there are any...
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His
I just bought the MSI panel antibodies (MLH-1, MSH2, MSH6 and PMS2) from
Ventana/Cell Marque and I'm running them on the Ultra and XT: and I'm having
trouble, as well, getting them to the desired staining for my pathologists.
I'm staining them at CC1 standard and antibody incubation time is 40
We are a GI lab and I do 3 levels on each block, 3 sections on each slide
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Allen
Sent: Monday, January 09, 2012 3:26 PM
To: histonet@lists.utsouthwester
riginal Message-
From: Andrea [mailto:abeharry...@gmail.com]
Sent: Tuesday, January 03, 2012 4:41 PM
To: Rathborne, Toni
Cc: Amber McKenzie; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: time off
We also have a policy on the number of people that can be off during peak
times. Our va
Those of you who are supervisors, how do you handle your co-workers asking for
time off? I have 2 employees that have asked off already (jan 3rd) for every
day they want off for the entire year! Do you grant them the days off since no
one else has asked off yet, or tell them it's not fair to
I know when you get a new instrument you have to validate every antibody
against previous stained slides on the original instrument, but what actions
must be taken for a new antibody? Besides, putting the protocol in and running
control slides.
___
Where can I get the article to show my billing dept/pathologists?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha
Ward-Pathology
Sent: Friday, December 30, 2011 1:13 PM
To: Webb, Dorothy L; 'histone
Where do you recommend buying lab chairs? I'm looking for some w/o arm rests,
solid black or tan, on rollers, etc..
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Would anyone be willing to share their SOP for validation? Thanks!
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Do you have to validate the neg mouse and rabbit?
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: Wednesday, November 16, 2011 4:42 PM
To: 'Kim Donadio'; Amber McKenzie; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Validation
Kim wrote: "Seems the actual amount of validation slides can vary with
different Medical directors( Pathologist)"
Yes, validation is s
Just wondering what y'alls opinion is on validation: I don't really understand
why optimization isn't enough. At that point, the pathologist has said what he
wanted the stain to look like, so why do 3-10 positive slides on the new
instrument to compare to previous slides from another instrume
I have my paraffin chambers set at 60 degrees (embedding center and processor)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
MacDonald
Sent: Friday, November 04, 2011 11:00 PM
To: Zoe rosa
Cc:
Can I do HistoQip if I am CLIA certified or does it have to be a CAP lab?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Sunday, November 06, 2011 2:17 PM
To: 'Janice Mahoney'; joellewea.
Our Peds Dr takes anywhere from 6 to 13 bx's, depending on if it's a lower or
upper GI or a double. Our other dr's take 0-6 bx's per patient depending on if
the patient has problems, if the dr see's something he wants to bx, etc...or
zero of everything looks normal.
-Original Message-
Go to www.ascp.org and look it up
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heath, Nancy L.
Sent: Thursday, October 13, 2011 5:35 AM
To: histonet@lists.utsouthwestern.edu
Cc: histonet-boun...@lists.ut
What about Alcohol & formalin from the processor? The diluted alcohol (100,
95, and 75%) and the alcohol from the cleaning cycle? Do you not pour that
down the drain with water? And, what about the H&E stainer...do you not pour
the bluing, hematoxylin, eosin and acid alcohol down the drain?
Hey, do y'all wash down your grossing table and soak your utensils at the end
of each day? What about the disposable knivesdo you throw them away after
each patient or at the end of each day? Thanks!
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If you only need to keep QC records for 2 years, is it the same for QA
records?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of H R
Sent: Wednesday, July 13, 2011 1:30 PM
To: histonet@lists.utsouthwestern.ed
Does anyone know if there's an AL state meeting this year?
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A GI lab in Ocean Springs, MS is looking for a part-time histotech to
work Fri night/Sat. If interested call 601-624-8592
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Does anyone use a QC form for the Gross Room? I was thinking of using
one to keep up with the cleaning of the grossing table...any
suggestions?
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What are you using to check your fume hood's with for QC purposes?
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I am updating my QC Manual and wanted to know if you QC your Processors
and Microtomes? These are the only 2 pieces of equipment that I
haven't been QC'ing.
Here's what I QC each month, am I covering everything?
1. Eye wash station
2. Fridge temp
3. 10%formalin
4.
Does everyone print out IHC run reports after each run? If so, how long
do you keep them and are the pathologists supposed to sign off on them
with their slides?
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In MS, it's a problem of not having anyone to fill the vacant positions. And
once you find someone, that person can pretty much say the hours she/he wants
and the pay b/c you have no other candidates to choose from.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
If anyone is interested in attending the annual Mississippi Society for
Histotechnology Symposium June 24-26th in Hattiesburg, MS, please
contact me directly and I can email you a program! We'd love to have
you :)
Amber McKenzie, BS, HT(ASCP)
MSH Secr
Back-up battery
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heather R
Sent: Saturday, April 23, 2011 1:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Processors and power outages
Wonde
My last CLIA inspection, I was told to check the temperature in the wax
chamber of my embedding station to compare against the temperature it
says it is...does anyone know of a good thermometer to purchase?
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I was wondering how long to keep all my QC log sheets...so CAP is 2 years?
Does anyone know about CLIA's rules?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
MacDonald
Sent: Monday, April 18,
Does anyone use any type of spray to keep the blocks from sticking to
the molds when popping the blocks out after they've been embedded?
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I recently got an Ultra about a month ago and doing test runs, I have
really enjoyed the continuous feed as well but I have a question...if I
deactivate a product from the Ultra's inventory list I notice it's still
in my XT's inventory list. So, I have to deactivate everything off of
each instrume
Job Opening in Jackson, MS:
If anyone wants to work nights as a bench tech embedding, cutting,
staining, putting cases together and filing slides at a GI lab in
Jackson, MS, you can fax me your resume at 601-326-3532. Thanks!
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Do pH strips go bad after a certain time period?
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I have to put my 2 cents in about all the statements on having an alias
name instead of identifying who each person is after each email...WHAT'S
THE BIG DEAL?? We are all supposed to be professionals asking each
other for advice/suggestions on the Histonet - who cares who each person
is? If I
Where do you guys buy chairs for your lab? Do they have to be any
certain type of chair?
Amber
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her. If anyone is planning on going to this class,
could you send me a copy of any handouts?
Thanks,
Amber McKenzie, B.S., HT (ASCP)
1405 N. State St., Suite 400
Jackson, MS 39202
(ph) 601-863-0388
(fax) 601-326-3532
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