Hi César we use mo-a-C4d, clone CL314 (Quidel A213) at 1.25 ug/ml (1:800) for cryosections of kidney biopsies (indirect immunofluorescence), it won't work on FFPE material (at least in our hands). For FFPE, Biomedica's rb-a-C4d (BI-RC4D) works fine at ca. 6 ug/ml (1:30) after HIER in a high pH buffer (Tris-EDTA, pH 9.0; Tris-Urea, pH 9.5). Hope this helps.
Best regards Andi Kappeler Institute of Pathology, University of Bern, Switzerland -----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Cesar Francisco Romero Gesendet: Dienstag, 24. März 2009 05:01 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] C4d Antibody Dear People from Histonet Could you share your experience with C4d Antibody ? I couldn't get any results with the mouse monoclonal from Quidel. I will try now the rabbit polyclonal ( C4dpAb )from Biomedica - Grouppe. I work in a Public Hospital with many kidney transplantations per year. Thank You in advance. César Romero Buenos Aires Argentina _________________________________________________________________ ¿Querés saber cómo va a estar el clima mañana? Ingresá ahora a MSN http://tiempo.ar.msn.com/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet