[Histonet] Mammary Tissue Processing & Sectioning Troubles

2017-09-27 Thread Andrea Calhoun via Histonet
Hi All! I'm working with primate mammary tissue that was given to us from a pathology group off campus. They say the tissue was fixed in NBF for at least two days, and sent to us in 70% EtOH, where it has sat for a couple months. We are treating these samples as we would with human tissue.

[Histonet] Using same DAB for multiple slide racks

2016-04-25 Thread Andrea Calhoun via Histonet
Hi Listers! Has anyone ever tried re-using DAB? Not like week or day old DAB, but using the same DAB solution for more than one rack of slides? Anyone know the length of time DAB is activated for before becoming inactivated, or quenched? Safe to do, if more H202 is added? After antibody