Hello everyone,
I recently received rat brain samples that were frozen in liquid nitrogen and
embedded in OCT. I sectioned them on the cryostat and they are coming out very
wrinkled, no matter what technique I use to pick them up. I would appreciate
any tips/tricks that anyone has to offer.
You can try removing two of the absolute alcohol steps, as it seems you are
using too much 100% etoh. Also, the tissue is probably dry from not being
infiltrated properly, so instead of doing 3 paraffin steps, you can try 1 step
for 1 hour to remove xylene, and then the second step over night.
Hello,
We are trying to order activated rat anti-mouse microglia antibody (1:200) from
BD Pharmingen but are unable to find the correct catalog number. Does anyone
know where I can find it to order it? Thanks.
Andrea
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