[Histonet] Help! Losing sections from Superfrost Plus Slides

Hi all, We have suddenly started losing tissue sections from our Superfrost Plus Slides. Our students have been cutting fixed, frozen, cryosections (20um) and thaw-mounting these onto Superfrost Plus slides. We have suddenly started losing lots of sections from these slides (again!!). The

[Histonet] IHC on frozen vs paraffin embedded tissue

Hi Everyone, I have a number of questions regarding paraffin embedding as I am a newbie to this type of tissue processing. I am wondering if anyone has any insight on doing IHC on frozen and paraffin embedded tissue for the same antigen. Is there much difference in the quality of the IHC,