Re: [Histonet] [EXTERNAL] Epredia xylene substitute

protocol. For standard processing we had/have two stations with 60 min each at 40 °C. For fat processing the time is doubled. That works for our specimens well enough. Gudrun -Ursprüngliche Nachricht- Von: Ann Specian via Histonet [mailto:histonet@lists.utsouthwestern.edu] Gesendet: Freitag

Re: [Histonet] [EXTERNAL] Epredia xylene substitute

. Will Cavett, II -Original Message- From: Ann Specian via Histonet Sent: Wednesday, April 24, 2024 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL][Histonet] Epredia xylene substitute We are thinking of changing from xylene to this substitute. Does anybody have any

[Histonet] Epredia xylene substitute

We are thinking of changing from xylene to this substitute. Does anybody have any Processing protocols using Epredia Xylene substitute that they could share? Sent from the all new AOL app for iOS ___ Histonet mailing list

[Histonet] Embedding

Can anyone let me know if CAP, New York State or ISO have any required credentials for someone to be able to embed human tissue? Sent from the all new AOL app for iOS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Clarapath SectionStar

Is anyone familiar with this new technology? Sent from the all new AOL app for iOS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Xylene Substitute Recycler Validation

We just received a recycler and would like to know if anyone has performed a validation on the recycler  prior to putting into use (separate from the processing validation).  If you have, is it possible to share it with me?  Thank you,  Ann  ___

[Histonet] Hologic PAP Imaging

Is anyone using a xylene substitute when staining their PAPs and imaging using the Hologic Imagers?  If you are, can you tell me which xylene substitute you are using (as long as it is FDA approved).  Have you had any issues?  Thank you, Ann ___

[Histonet] Embedding

Is anyone aware of any NYS/CAP/CLIA regulations regarding credentialing required to perform embedding in NJ?Thank you, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Change in Histotech training/educational requirements

Can someone tell me if there has been a change in the training and education requirements in the New Jersey/New York area for Histotech’s. Were there any regulatory changes? Sent from AOL Mobile Mail Get the new AOL app: mail.mobile.aol.com ___

[Histonet] Paraffin block storage

Does anybody have a reference as to the proper temperature to store paraffin blocks. What is the highest temp permitable. Sent from AOL Mobile Mail Get the new AOL app: mail.mobile.aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Proscia

Has anyone used Proscia for digital imaging? Comments? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Billing for iuds with tissue

Can someone tell me the correct way to bill an IUD that has tissue. If there is no tissue then we just bill cpt code 88300. If there is tissue should you bill 88305 or 88300 and 88305? ___ Histonet mailing list

[Histonet] Fuelgen stain for urine cytology

Does anyone have a staining procedure for the fuelgen stain for urine cytology that they can share Sent from AOL Mobile Mail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Acid Hematoyxlin stain for Urine

Does anyone have a procedure for an acid hematoxylin stain for urine cytology? I believe Bostwick used to/does perform it.. Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Sakura Automated Embedding Center and Express processor

Is anyone familiar with the Sakura Automated Embeddingcenter or their Express processor? If so, can you let me know yourthoughts. Thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Histology school in NJ

Does anyone know where to find the requirements to start aschool for Histotechnology in nj? Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] microtome validation

Has anyone had to validate a microtome (manual/automatic). If so, how did you do it? Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] guidelines for using digital images as a primary diagnostic tool

Does anyone have any information in regard to using digitalimages as the primary diagnostic tool? Thanks, ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Cassette Printers

I am looking to purchase a cassette printer. Does anyone have any suggestions as to which are the best and which should be avoided? Thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Tissue processing validation

We are moving out Peloris tissue processors to another room, so we have to validate. I was wondering what other labs do as a validation for this type of move? Do you run tissue on every progam utilizing each processor for at least one of the protocols? Any help would be appreciated. thanks,

[Histonet] Lifting sections off of slides

Does anyone have a procedure where in you can lift the stained sections off of a slide and place them on another slide? Sent from AOL Mobile Mail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Post fixing IHC slides in formalin

Does anyone post fix their IHC slides in formalin in an effort to try to reduce tissue loss? If so, does anyone have a protocol for this that they have used and have seen good results? If you have any other suggestions which can help to reduce tissue loss during IHC staining, I would love to

[Histonet] Slide oven temperature

Can someone tell me the range you set your slide drying oven at and what you base it on. thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] SOP for submitting slides for HQIP

I have to write an sop for submission of slides for HQIP. Does anyone have one they can share? Thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Price for preparing IHC slides

Can anyone tell me the average cost for preparing an IHC slide? thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Radiation SOP for Histology

I need a radiation sop specific to Histology. Does anyone have one they can share? thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] ANP 23045 The performance of all instruments and equipment is verified before use.

Has anyone written a procedure for this new checklist item? If so, what procedure are you using to verify equipment/instrument performance prior to use? Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Cell Block Preparation

I am getting complaints in regard to insufficient cell blocks. We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper. Does anyone have a more current technique which renders better cellularity? Also, do you know which renders a better cell block: a

[Histonet] Cryostat cleaning

ANP.12087 states that the cryostat needs to be defrosted and wiped clean with a tuberculocidal. Can someone tell me what they use that meets the CAP criteria listed above. thankis, ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Cryostat Cleaning

Can someone tell me the CAP approved chemical that can be used to clean the cryostat? thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Voice Recognition Systems

I am looking for a voice recognition system for the gross room. It needs to be a system that will interface with our LIS. Does anyone have any recommendations in regard to vendors? Thank you, Ann Specian ___ Histonet mailing list Histonet

[Histonet] Negative Controls in IHC

I have a question in regard to eliminating the use of negative controls when using a non-avidin-biotin detection system. Do you not feel that negative controls may still need to be run in tissues which are likely to be pigmented such as lymph node, skin and liver? We were thinking to

[Histonet] Embedding

We are having a problem with floaters in our blocks which occur during embedding. We have multiple forceps which are placed in heated wells and each cassette is embedded with a new forcep. We also wipe with a gauze, but we are still getting floaters embedded in the cassette from time to

Re: [Histonet] Embedding

we clean them at the start of our shift, but not during embedding. do you clean them during embedding too? -Original Message- From: Norton, Sally sally.nor...@seattlechildrens.org To: 'Ann Specian' thisis...@aol.com; histonet histonet@lists.utsouthwestern.edu Sent: Fri, May 25, 2012