[Histonet] paraffin block disposal in NYS

be discarded as solid waste. Annette Featherstone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Freezing rodent brain

How is everyone freezing hippocampus sections? The current method being used seems to be causing a lot of artifact. Thanks Annette Featherstone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman

[Histonet] The Milestone PATHOS

Has anyone used or is currently using The Milestone PATHOS for tissue processing? What are your results, pros and cons? Would this be good for breast biopsies? Thanks Annette Featherstone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] RAGE and AGE

Is anyone using RAGE or AGE antibody successfully? If so would you be willing to share vendor and data? I am also looking for a Tunel Assay kit, what would any of you recommend? Thanks, Annette Featherstone ___ Histonet mailing list Histonet

[Histonet] CD31 in rats

Is anyone using CD31 antibody in rats, if so, what vendor and what protocol. thanks Annette Featherstone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Sheep Lung

We will be cutting frozen sections on sheep lung and I was wondering if anyone has a method to produce good quality sections. Are you injecting anything in the lung prior to sectioning? Annette Featherstone ___ Histonet mailing list Histonet

[Histonet] MCP-1

Is anyone working with MCP-1 Armenian hamster, from Biolegend? This is for FFPE tissue. The secondary is anti-Syrian hamster. Any help would be appreciated. Annette Featherstone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] F4-80 anti mouse

Is anyone out there using F4-80 anti mouse (Biolegend) for macrophage. What is your protocol for Paraffin embedded sections? Any help will be appreciated. Currently we have no staining at 1 to 100 for the primary and antigen citrate buffer. thanks Annette Featherstone

[Histonet] Adhesive for immunos

We have used 5% Tite-bond glue( from the Hardware store) on slides with great success. Just take a Kimwipe and wipe it evenly over the slide and it holds the tissue very well. Annette Featherstone, Supervisor of Pathology and Anatomical Sciences The University of Buffalo Phone:716-829