RE: [Histonet] TTF-1/background staining

Hi Paula I do not believe that RTU Abs really exist. No company can produce an antibody that is suitable for use by all laboratories taking in to consideration how tissue can be handled differently from lab to lab. I have used many RTU Abs which I have had to dilute to get optimal staining

Re: [Histonet] lost tissue

Hi Christine You have had some good suggestions to date. Also to consider if you are using the Peloris racks with the individual spaces for cassettes I have seen this happen when cassettes are inserted into the racks. As most people load cassettes with the patient details facing up, if

[Histonet] RE: Bag Sealing system

Hi Loralee Try a butcher's supply store. They sell devices they use for vacuum packing meat. By removing the air from the bag the evaporation of formalin is almost zero and the specimens will remain intact for many years. The bags are also more hardy than those sold with basic bag sealers.

Re: [Histonet] HM 500 M cryostat issue

Hi Andrew I have not used that model cryostat so this may not be the answer but I have experienced this on other cryostats when the microtome has been removed for cleaning and decontamination and reinstalled with the handle in the incorrect position resulting in the counterweight being

Re: [Histonet] decontamination of cryostat

Hi Annie If you look up this article or many others like it on the net the simplest and most effective disinfectants are 70% alcohol or one of the aldehydes for most organisms remembering that you are only disinfecting a hard surface with no visible volume of liquid containing organisms. We

RE: [Histonet] ER clone 1D5 or SP1 ?

Hi A large number of studies have been performed which show that 6F11 will stain a number of tumours that 1D5 does not and I know a number of labs who have moved away from 1D5 because of this. Google 1D5 and 6F11 and you will find the articles. regards Tony Tony Reilly B.Sc.

Re: [Histonet] Cleaning VIP Processor containers

Hi Brandi Do you do hot water flushes? If not the film will be a build up of the buffer salts from your formalin. To flush replace your formalin containers with hot water and write a program to run each container for 10-15 minutes. This will not only clean the retorts but the fluid lines as

Re: [Histonet] Processing lavages into cell pellets...

Hi Jennifer I realise there are products like Histogel about of which I have no knowledge however I have been using agar for over 30 years because I can get it easily and gratis from our microbiology department. My experience is to spin down the cells, remove the supernatent, then add

Re: [Histonet] tb-controls selfmade

Hi Gudren I have not done this with TB but I have with other bacteria to produce controls for gram stains. You need to have the tissue in a nutrient broth appropriate for the organism. You need to consult with a laboratory that specialises in Mycobacteria to find the appropriate medium to

Re: [Histonet] alcian blue van gieson stain

Hi Margaret You perform the Alcian Blue first but as with Movatt's pentachrome you need to treat the sections after AB with alcoholic ammonia for an hour to convert the AB to Monastral Blue as the AB is soluable in picric acid and the Monastral blue resistant. Let me know if you need a method

RE: [Histonet] Double labeling with antibodies that need differentfixatives

Hi Phebe The first place to look when working up Antibodies is the Specification sheet from the manufacturer. If you look at the BD sheet for their CD31 Immunohistochemistry is recommended for Zinc fixed paraffin sections and acetone fixed frozeb sections but not recommended for formalin

RE: [Histonet] Double labeling with antibodies that need differentfixatives

Hi Phebe The first place to look when working up Antibodies is the Specification sheet from the manufacturer. If you look at the BD sheet for their CD31 Immunohistochemistry is recommended for Zinc fixed paraffin sections and acetone fixed frozeb sections but not recommended for formalin

Re: [Histonet] Polarizing filters

I worked with a pathologist many years ago who would use one piece of polarising glass on the light source and wear his sunglasses. It worked fine. regards Tony Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital

Re: [Histonet] water on slides?

If your solutions are clean the cause may be that the level of the water wash station on your stainer is higher than the level of the subsequent alcohol container which means that some water will not be removed from the top of the slide. This is common with running water washes on stainers as

Re: [Histonet] Pathology to OR Communication System

Hi Luke A simple cheap solution would be to buy telephones for the OR and the lab that have speaker phone capabilities. No expensive purchase cost or installation required. regards Tony Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15

Re: [Histonet] (no subject)

I have used DAKO Target Retrieval in the past and it is definitely possible to re-use the solution. The important thing to do is to check the pH each day before use as this is the best indicator that the solution needs to be changed. It has bee an while since I used it but from my shaky

Re: FW: [Histonet] (no subject)

Of Anthony Reilly Sent: Wednesday, 15 April 2009 10:31 AM To: histonet@lists.utsouthwestern.edu; jeff Subject: Re: [Histonet] (no subject) I have used DAKO Target Retrieval in the past and it is definitely possible to re-use the solution. The important thing to do is to check the pH each day before use

Re: [Histonet] Strange circles in IHC slides

Hi Looking at the images this appears to be the prozone effect which is caused by the primary antibody concentration being too high and has been well reported in both immunohistochemistry, immunology and serology articles. Do a google serch for prozone phenomenon and you will find an

[Histonet] Calcium salts

Hello I am trying to differentiate Calcium Phosphate from Calcium Carbonate which both stain with the von Kossa method. Is there a pretreatment for which only one is soluble that could be used to differentiate the 2 components. thanks Tony Reilly Chief Scientist Anatomical Pathology

Re: [Histonet] overseas jobs

Hello Joe This is an interesting question following on from all of the discussion recently about uncertified techs. In Australia you need tertiary qualifications to work in a technical position in Histology. Histologists are regarded and paid at the same level as all other staff working in

Re: [Histonet] equation problem PLEASE help

Hello Eva Put simply 1nmol is 10-9 moles Therefore 10nmol is 10-8 moles 1 mole = 1,064g Therefore 10-8 moles = 0.1064gor 0.01064mg Therefore your working solution of 10nmol/ml = 0.01064mg/ml Your stock solution is 1mg/ml Therefore your dilution factor is 1.0

Re: [Histonet] equation problem PLEASE help

Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au Anthony Reilly tony_rei...@health.qld.gov.au 8/01/2009 12:39 pm Hello Eva

Re: [Histonet] Slide drying

Hi Erin While the request from your pathologists seems unreasonable from my experience the following steps will give the best results. Use a rapid drying mountant many of which are on the market. Consult your local suppliers. Dry in an incubator as you have been doing but when you remove