Hi,
I am wondering if anyone of you have a written SOP on 'Cryosectioning of animal
tissues implanted with human cells that are totally not screened for Hep B, HEP
C, HIV, Mycobacterium tuberculosis and other adventitious agents' . If you have
one written, would you be kind enough to share
Hi,
I am in a research lab and currently we are looking into buying a good cryostat
to work with human samples. I have always used a Leica model and currently we
own a Leica 3050S. We would like get one that comes with decontamination
feature (UV lamp etc...). I would like to know your
She is right.
Day 0 is today (the same day you start the run) -day 1
Day 1 is tomorrow start the run the next day,- day 2
Day 2 is the day after tomorrow - day 3
Day 3 is the following day -day 4 and so on
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I have sectioned some formalin fixed, paraffin embedded pig skin blocks in the
past, but never the fresh frozen pig skin blocks. The samples were frozen by
the researcher in liquid nitrogen, but not covered in cryomatrix and have been
stored in the deep freezer for over a year. I have now
[mailto:jo...@mcclainlab.com]
Sent: March 22, 2011 9:22 AM
To: Bascaramurty, Saro
Subject: RE: [Histonet] Frozen sectioning of pig skin
Did you thaw the tissue before you embedded in media? If not, try it.
I use NEG-50 from Richard-Allan Scientific to embed frozen tissue. I have
found