[Histonet] Written SOP

2011-07-11 Thread Bascaramurty, Saro
Hi, I am wondering if anyone of you have a written SOP on 'Cryosectioning of animal tissues implanted with human cells that are totally not screened for Hep B, HEP C, HIV, Mycobacterium tuberculosis and other adventitious agents' . If you have one written, would you be kind enough to share

[Histonet] Could you please recommend?

2011-06-29 Thread Bascaramurty, Saro
Hi, I am in a research lab and currently we are looking into buying a good cryostat to work with human samples. I have always used a Leica model and currently we own a Leica 3050S. We would like get one that comes with decontamination feature (UV lamp etc...). I would like to know your

RE: [Histonet] Question about delayed start in Leica Tissue Processor

2011-04-18 Thread Bascaramurty, Saro
She is right. Day 0 is today (the same day you start the run) -day 1 Day 1 is tomorrow start the run the next day,- day 2 Day 2 is the day after tomorrow - day 3 Day 3 is the following day -day 4 and so on -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Frozen sectioning of pig skin

2011-03-22 Thread Bascaramurty, Saro
I have sectioned some formalin fixed, paraffin embedded pig skin blocks in the past, but never the fresh frozen pig skin blocks. The samples were frozen by the researcher in liquid nitrogen, but not covered in cryomatrix and have been stored in the deep freezer for over a year. I have now

RE: [Histonet] Frozen sectioning of pig skin

2011-03-22 Thread Bascaramurty, Saro
[mailto:jo...@mcclainlab.com] Sent: March 22, 2011 9:22 AM To: Bascaramurty, Saro Subject: RE: [Histonet] Frozen sectioning of pig skin Did you thaw the tissue before you embedded in media? If not, try it. I use NEG-50 from Richard-Allan Scientific to embed frozen tissue. I have found