RE: [Histonet] Pathology Protocols

54702 | mayoclinichealthsystem.org -Original Message- From: Douglas Porter [mailto:doug.por...@caplab.org] Sent: Tuesday, March 11, 2014 10:55 AM To: Bauer, Karen L.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pathology Protocols The key to your plan is having pathologists that are willin

[Histonet] Pathology Protocols

Hi all, Are there any AP labs that are totally paper free during slide diagnosis? We have the Vantage system with Sunquest CoPath. We still keep the paper specimen requisition with the specimen container during grossing. After digital gross dictation, the req slips are given to the transcript

[Histonet] RE: Fungus Controls

Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Thursday, February 20, 2014 2:57 PM To: 'his

[Histonet] Fungus Controls

Hi all, We are in need of some Fungus controls. Anyone have any extras to spare? Will do a trade... if we have anything available for you. Thank you very much, Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205

[Histonet] RE: Ventana LEAN team

Hi Amber, As part of our Vantage implementation process, a workflow consultant initially came out to look at our processes. He was very nice and had a lot of great suggestions. He was never pushy and I had control of what I wanted to change or not change. He basically was there to see how th

[Histonet] RE: Acccessioning area/Grossing area

We do it that way as well. It seems to flow quite nicely and it works well for us. Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Cl

[Histonet] Purple/Blue haze on ISH

Hi all... sending out for a colleague... After implementing the Vantage system, a lot of their ISH slides have a purplish to bluish haze on the slides. Not all of them, but some and it seems to be consistent. No problems before Vantage. They were using the longer labels from Ventana for labe

RE: [Histonet] Specimen Tracking Systems

Message- From: Morken, Timothy [mailto:timothy.mor...@ucsfmedctr.org] Sent: Friday, November 01, 2013 11:23 AM To: Bauer, Karen L.; 'Jesus Ellin'; 'WILLIAM DESALVO'; 'Matthew D. Roark'; 'histonet' Subject: RE: [Histonet] Specimen Tracking Systems Karen,

RE: [Histonet] Specimen Tracking Systems

Jesus, Tim, and Bill... Wonderful communication about specimen tracking, bi-directional capabilities, and creating a histology lab that will be effective in the years to come!! I loved reading your information! We also have the Vantage system and it's helped us decrease our slide labeling err

RE: [Histonet] RE: MOHS IPs

2013 9:36 AM To: Barbara Tibbs; Bauer, Karen L.; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] RE: MOHS IPs I agree with Barbara try fixing in cold acetone/ethanol mix even = just for a couple of minutes then go directly to buffer do not dry after=x, I woul

[Histonet] MOHS IPs

Hi all, We are in the process of validating some antibodies in our MOHS lab. The Melan A (Mart 1) antibody is working well, but it could be darker. We will be increasing the Ab incubation time to see if that will help. As for the Pan Keratin, we cannot get it to work at all. We use Protease

[Histonet] MOHS Tech

Hi, We are looking for MOHS tech and have an applicant that is not a certified histotech. Our MOHS techs are expected to gross tissues, which requires a certified tech for high complexity testing... according to CAP regulations. I was wondering what other MOHS techs are doing that are not cert

[Histonet] Tissue Tek Xpress

Hi, My future plan for our lab is to purchase a Tissue Tek Xpress for continuous processing. I'd like to hear from Xpress50 users and Xpress120 users. What are your processing schedules? What do you process throughout the day? Would you recommend two 50's instead of one 120? Any thoughts

[Histonet] Vantage Printers

Hi Histoland, For those of you that are using the Vantage from Ventana, what cassette printers have you found work the best? Thanks much!! Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor - Pathology Department MOHS Lab Supervisor - Dermatology Department Mayo Clinic Health System in

RE: [Histonet] time off

Hi Amber, I have a Holiday Rotation List that I use for my staff. I started the list off with "seniority", but then everytime a tech uses PTO around a holiday (either before or after), they go to the bottom of the list for that holiday. This ensures that all techs have a chance to take off and i

RE: [Histonet] Ventana Eber Probes

Hi Andria, This is our EBER protocol that we are using on our XT... With great success. Using the XT ISH Open Probes - Chromogenic procedure... Depar Enzyme: ISH Protease 3 for 12 minutes Probe HybReady Probe Auto Dispense: Then pick the probe that contains EBER Denature: 60 degrees C for 4

RE: [Histonet] MOHS daily flow...

- From: Bartlett, Jeanine (CDC/OID/NCEZID) [mailto:j...@cdc.gov] Sent: Tuesday, July 26, 2011 12:53 PM To: Nicole Tatum; Bauer, Karen L.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] MOHS daily flow... That is very interesting. A friend had a procedure done last week and they kept him on

[Histonet] MOHS daily flow...

Hello, This is for those techs that assist in the MOHS lab... What is your daily flow for the work performed in your lab? How are patients usually scheduled? (Are they staggered? All come in at approximately the same time so 1st layers can be taken right away?) How often are you receiving p

RE: [Histonet] Assisting with Autopsies

Hi Amy, We do about the same number of autopsies per year... Only on in-house patients and they have to be physician requested, no family requested. We used to hire a diener to come and do them and the techs would assist. None of the techs cared to do that, so I usually volunteered, since I actua

[Histonet] Continuous processing

Hi all... I'd like to get a quick reply on what everyone considers is the best continuous processor out on the market. I'd like to start processing the small biopsies continually throughout the day and I've been thinking about the Tissue-Tek Xpress or Xpress 50. Comments and suggestions are g

[Histonet] Controls for IPs

Hello, It seems we go back and forth between multi-tissue control blocks and single tissue control blocks when it comes to immunostaining. In the past we used single blocks and still continue to use some for the IPs that are rarely used. Then, switched to creating multi-tissue blocks for eas

RE: [Histonet] (no subject)

Rhonda, We keep our empty biopsy containers for one week and then dispose of them in a red bag. There are no "recommendations" that I know of, but we started doing it because there was a mix up with a biopsy case and we couldn't figure out if the nurse had put the specimens in the wrong labeled c

RE: [Histonet] alcohol monitoring

Hi Cristi, We have been monitoring alcohol fumes for many years. Our hospital safety officer orders badges and we wear them on our lab coats for 15 minutes and another one for 8 hours. Only one tech needs to wear them in the lab, not everybody. We usually do it on a day where we will handle

RE: [Histonet] Switching Back to Formalin

Don't dispose of it... Recycle it. Creative Waste Solutions has a nice "Green" recycler for smaller labs, but we're using the BR Instrument for our lab. I never imagined that we would recycle our formalin, but when we tried out the BR, I was very impressed. We've saved so much by not having to c

[Histonet] Linear Slide Stainers

Hello... I've been told that the GLX and SLS Linistat linear slide stainers are being discontinued. I was going to order a GLX for our new Mohs lab, and now I'm unsure what to purchase instead. What are others using? Do you need a fume hood? If I go formalin free and xylene free, do I need a

[Histonet] Mohs Histotech Needed

Hello all... We have the following position open: "Mohs Histotech needed for a new Mohs surgery practice with an established Mayo Health System facility. The position is full-time, split equally between Mohs and a traditional hospital lab. ASCP registry/eligibility required. Mohs experienc

[Histonet] IgG4 antibody

Hello, I'm trying to find an IgG4 antibody to use on our Ventana Ultra IP stainer. Is anyone out there using this antibody on their XT or Ultra? If so, could you please share with me the vendor you go through and what you are using for a protocol? Thanks much, Karen Karen L. Bauer HTL (ASC

[Histonet] Microm vs Leica

Hello, We are starting up a new Mohs lab and there have been discussions on what brand of cryostat to purchase. The new doc would like us to get Microm cryostats, but I would like to stick with the Leica, since we us them in the Histology lab and know and trust them. They have been workhorses f

RE: [Histonet] Automated H&E stainers

Brett, We purchased the Tissue Tek PRISMA with the attached film coverslipper a year ago and LOVE IT!! Was a little nervous switching from the glass coverslips to the film, but the docs love the slides and we have had no problems. Very easy to use and we like the fact that we can file the slides

[Histonet] Combining Cytology and Histology

ogether. One of our docs wants to have everything from Histology and Cytology on one report for the patient. Is this possible? Can Cytology still result the case if we have one accession number for everything? Any help or ideas on this issue would be greatly appreciated. Thanks much! Karen

[Histonet] Online SOP's

Hello, Does anyone have only procedures online? All of ours are online, but we still keep a paper copy of each in a manual in the lab. I would like to get rid of the manual, but then how do you record pathologist sign-off on all new or revised procedures? I'm curious to hear what other labs a

RE: [Histonet] perplexed

We also had this happen, and the red control box marking was on the underside of the slide. Seems that when the slides are packaged in the box, the front part of the slide is pressed with the red markings on the back of the slide in front of it. This creates an invisible barrier on the non-marked