[Histonet] Histology temp position available at Ionis Pharmaceuticals in Carlsbad, CA

We will soon have a temp position available in our very busy histology laboratory at Ionis Pharmaceuticals. If you are interested, please forward your CV to me. Have a great day, Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Ionis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct Carlsbad,

Re: [Histonet] Specimen Accessioning .....

Same here. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: Weems, Joyce K. [mailto:joyce.we...@emoryhealthcare.org] Sent: Wednesday, June 03, 2015 1:14 PM To: 'Cartun,

[Histonet] Thank you everyone who responded to my service equipment request

Thank you! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Who are you guys using to service equipment in the San Diego area?

Who are you guys using for servicing different equipment like microtome, cryostat, stainers in the San Diego area? Thank you in advance, Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371

Re: [Histonet] Hand-on wet IHC Workshop for Researchers

Could you please send some more information on that and where to register? Thanks, Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: IHC Workshop

RE: [Histonet] Cheap Disposable Blades for Facing In

We are doing the same. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf

[Histonet] RE: Rubber mats for pinning specimens

Have you tried to pin on large cork sheets? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] RE: CD31 in rabbit tissue

We use CD31 from Spring BioScience (SP38). Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] RE: whole slide scanners

I totally agree with both of you. We upgraded our Aperio (Leica) ScanScope XT to the new AT, which holds 400 slides and is much faster than the XT. Both can also scan 2x3 slides manually. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct.

[Histonet] RE: Cassette Printer

Yes, we have one. Our first one was a lemon, but was replaced at no cost. Ever since then we had no problems whatsoever with it. We like it, because it has a small foot print and the printing is very clear, even with bar codes. The cons might be, that you have to unload the slides after a while

RE: [Histonet] Peggy Wenk's passing

Lee, I am so sorry to hear about your loss, and a huge loss and void to the world of histology. My sympathies go out to you and your family. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371

[Histonet] RE: BrDU

Hi Luis, We are using the BrDU In-Situ Detection Kit #551321 from BD Biosciences with good luck. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From:

RE: [Histonet] Aperio slide scanner

Jan, We like ours a lot. And I agree with all that Liz mentioned. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Microtome

Leica 2125 Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Sent:

[Histonet] RE: Wrinkles on Glutaraldehyde Fixed Tissue

Why do you use glutaraldehyde for eyes? Davidson's is a much better fixative for eyes. With Davidson's you have two fast penetrating ingredients and one slow acting fixative. Glut is only a fast fixative. Besides, the best institutions who deal with eyes only use Davidson's. Just my thoughts.

[Histonet] RE: Recommendations cryostat

Leica! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

[Histonet] RE: cryostat design

Leica has a more ergonomically correct cryostat, which sits lower and your feet are on the ground. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From:

[Histonet] RE: Immunocal

I had good luck with Chelator Cal from BBC Biochemical. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] IHC tutorial in California

http://innvx.com/workshop.html Here you go! Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] CD68 or F4/80

We had success with CD68 on mouse tissue from Boster cat# PA1518. For F4/80 we use the rat anti-mouse antibody from eBioscience cat# 14-4801. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original

RE: [Histonet] Rodent eye paraffin microtomy

Let the eye soak in ice water before cutting for a very long time. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] wax on the floors

Paraffin scraper from Mercedes Medical http://www.mercedesmedical.com/default.aspx?page=item+detailitemcode=MKL+ML1138 Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From:

RE: [Histonet] RE: Yahoo link

Good one, Pam! :-) Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

RE: [Histonet] Chris van der Loos

Very sad news indeed. He will be missed dearly! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Aperio ScanScope XT vs. ScanScope AT Turbo

Hi histonetters, I would like to hear from people who made the switch from the original Aperio ScanScope XT to the new ScanScope AT Turbo. We are in the process to possibly change over. My questions are how you like it overall, if you experience any major problems/issues, how much faster it

[Histonet] RE: Microtomes

Leica. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski

RE: [Histonet] QIHC

Dako IHC hand book. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

RE: [Histonet] Preventative Maintenance on reagent and formalin recyclers

Annually. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim

RE: [Histonet] Equipment selection

As far as stainer and coverslipper, I think it comes down to personal preference. I've used both, and I think both are very good. When it comes to Immunostainer, I'm familiar with Ventana, Dako, Thermo Scientific Autostainer (Labvision) and Bond Max. I do not like the Dako/Thermo platform at

[Histonet] Monkey processing schedule for brain and spinal cord

Would someone be so kind and share with us their monkey processing schedule for brain and spinal cord, please? Thank you in advance, Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371

RE: [Histonet] RE: Xylene Substitutes

At another place I've worked for, we used Propar for animal tissues and it worked well. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From:

RE: [Histonet] IHC after EDTA decalcifying

Hi Colleen, We use ChelatorCal from BBC Biochemical, which contains EDTA. It is very gentle and retains antigenicity for IHC stain. We really like it. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371

RE: [Histonet] processing eyes

Patsy, 18-24 hours in Davidson's followed by 70% ethanol prior to processing. Some people also store the eyes in 10%NBF. I will attach a schedule for you off line. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010

[Histonet] RE: Coverslipper recommendations/ feedback

We like our Leica coverslipper. I guess it also depends on what kind of automated stainer you have, if you want to have continuous motion. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original

[Histonet] Contract laboratories for 2 x 3 brain sections; cutting, staining, possibly scanning

Hello histonetters, Who do you use for large brain slides (monkey, pig) to do the studies? Contract labs are welcome to contact me too. Thanks, Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371

RE: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides

We do IHC by hand most of the times.. http://www.newcomersupply.com/products/slide-staining-trays-ihc Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From:

[Histonet] RE: HE staining question

Hi Brett, That the HE staining looks faded after storage in 70% seems very unlikely. We do this with rat and mouse tissues all the time. Did the other lab use a different Hematoxylin, Eosin, or used a different staining protocol on the autostainer? Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC

RE: [Histonet] Who is scanning thieir HE's slides for Path.

Aperio. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston,

[Histonet] RE: Primera slide printer

: Wednesday, April 17, 2013 9:25 AM To: Blazek, Linda; Bea DeBrosse-Serra; 'histonet@lists.utsouthwestern.edu' Subject: RE: Primera slide printer Bea, Ditto what Linda said! Lori -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun

[Histonet] Primera slide printer

Hello histonetters, What kind of slides, from which vendor, do you use with the Primera slide printer? Do they need to be clipped corners? Are you happy with the slide printer? Does it have major issues? Any information would be greatly appreciated. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC

RE: [Histonet] IHC pipettes:

Eppendorf and the 4 different sizes, that Liz mentioned. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Test

___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] standard protocol

I sure have never heard of a standard protocol like this with any pharmaceutical company I have ever worked for. This is disturbing! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message-

RE: [Histonet] OT: Hi Histonetters!! I have some news I would like to share!

Happy anniversary! :) Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

RE: [Histonet] Which fixative for eyes

Davidson's fixative. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

RE: [Histonet] Looking at cryostats

Leica Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

RE: [Histonet] validation runs

Yes Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Melissa

RE: [Histonet] Can anyone recommend a good Rabbit Isotype Control antibody

Jackson Immunoresearch cat. # 011-000-003 or from VWR cat # 100181-130. Much cheaper Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From:

RE: [Histonet] Microtome feedback

(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: Ian R Bernard [mailto:ibern...@uab.edu] Sent: Saturday, August 18, 2012 3:53 AM To: Bea DeBrosse-Serra; Fred Underwood; Histonet Subject: RE: [Histonet

RE: [Histonet] Microtome feedback

Fred, The Leica RM2255 all the way! Go Dayton! Bea From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fred Underwood [funderw...@mcohio.org] Sent: Friday, August 17, 2012 12:48 PM To: Histonet

[Histonet] RE: Dewaxing offline

I was wondering about that myself. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] disposable blade holder

Check with your local Leica rep. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

RE: [Histonet] (no subject)

It all depends on how long you want to store the slides. The tape has a tendency to come off, whereas the glass slides, if covered properly, can be stored indefinitely. The glass coverslippers have improved quite a bit, and there are several vendors out there who have them. We have one here,

RE: [Histonet] automated H+E and cover slipper

I second on that! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

[Histonet] RE: Re: Methanol in H2O2 explanation

And the reason for that is...??? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Thank you for CD40 suggestions!

Thank you everybody who gave me excellent suggestions and feedback on CD40 on frozen sections, especially Gayle! I will keep the individuals posted on how everything goes. Thanks, Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad,

[Histonet] Need help with CD40 on frozen sections

Histonetters, I need some help with CD40 on frozen sections (spleen, non-treated). I cut my frozens, let them briefly air dry and keep them at -20ºC. When I use them for staining, I air dry them, fix them in cold acetone for 5 minutes and start the staining process. I use the primary AB from

RE: [Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates

We use Jackson Immunoresearch for our secondaries and the Biocare Alk. Phosph. Kit or the DAKO DAB plus kit. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From:

[Histonet] RE: Muscle Biopsy Procedure

Ian, Check with Children's Hospital in Cincinnati. The Pathology Department there performs a lot of muscle biopsy procedures and gets a lot of outside biopsies as well. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010

RE: [Histonet] QIHC exam

DAKO IHC guide book. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

RE: [Histonet] microtome

Leica 2255, all the way. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf

RE: [Histonet] Refrigerant for Cryostat

Mikron. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eric Baltazar

RE: [Histonet] H E Staining

Sheila, We are using the reagents from Leica, formerly Surgipath, Gill's II Hematoxylin (01520) and Eosin (01520) on our Leica Autostainer. But there are many other vendors that are very good too. Way back when, we used to make our own hematoxylin and eosin. But it really is so much easier to

RE: [Histonet] commercial antibody diluent with carrier proteins SUGGESTIONS?

I like the DAKO antibody diluent. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2588 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]

RE: [Histonet] blocking endogenous activities

The majority of the time we do it after retrieval. I believe you can do the hydrogen peroxide block at any point, as long as you do it before the DAB. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2588 Gazelle Ct. Carlsbad, CA 92010 760-603-2371

[Histonet] Morse fixative/solution

Hi histonetters, Does anybody know, if Morse fixative/solution is commercially available? Thanks in advance, Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2588 Gazelle Ct. Carlsbad, CA 92010 760-603-2371

[Histonet] RE: Cutting speed

Teresa, I totally agree with all the advice Toysha and Kim gave you. The cutting speed will come with time and experience. Even though in a clinical environment everybody seems to push speed, the quality of the slide should not suffer. My thoughts are, to take your time to produce good

RE: [Histonet] ?QIHC

Great advice! I second that! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

[Histonet] RE: Automated coverslipper with Ventana labels

Hi Teri, And welcome to San Diego :-) My experience is, that you cannot leave the slides with the Ventana labels sitting in xylene for too long before coverslipping. I believe it is just how these labels are and doesn't have much to do with the coverslipper itself. Bea Beatrice

[Histonet] RE: Please remove my name

If you want to be removed from the list, you have to do it yourself by going to the website below. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -Original Message- From:

RE: [Histonet] (no subject)

Do you coat the muscle biopsies with talcum powder prior to freezing? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Histotechnician requirements in Europe

I think it will greatly depend on the country. I received my certification in Switzerland, which actually is the licensure of Biology Laboratory technician. It was a three year apprenticeship (I believe it is four years now) with an accredited facility (mine was the Animal Pathology of the

RE: [Histonet] Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4%

Formaldehyde is the concentrated form and formalin the diluted form of formaldehyde. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -Original Message- From:

RE: [Histonet] Glass Coverslipper

I second that. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

RE: [Histonet] immunohistochemistry: manual staining

It is discontinued. I went on line, and that is what it said. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] RE: Floater Problem

Are gloves being worn during the whole process, including slide printing? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Automatic HE stainer with coverslipper (glass)

Leica...all the way! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

RE: [Histonet] RE: Embedding process improvement and competencyassessment

I heard of a lot of cheating as well. People paid others to do the blocks and staining. How good does it do? In the end, these people are cheating themselves. Very sad! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008

RE: [Histonet] Embedding process improvement and competency assessment

I have to agree with René. I have worked under registered histologists and MT's. And under certain circumstances it is EXTREMELY hard to make a non-histologist to understand what our needs are. But at least it appears to me, that you want to learn and are open to understand the histology way.

RE: [Histonet] your opinion on microtome

My favorite is the Leica RM2255. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] which conferences?

NSH. Nsh.org Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

RE: [Histonet] Formalin down the drain??

I totally agree, under any circumstances, whatever the State law defines, we should not dump formalin, xylene or alcohols down the drain. And even though California has very strict rules, it even seems to differ from county to county. In one of my past jobs we were allowed to dump the formalin