Hi,
I have been staining fish tissues fixed in Davidsons with H, and the
researcher would like the eosin to be more intense. Our standard protocol
works well for our own tissue, but the fish look much more washed out. I am
using alcoholic eosin Y, have tried both water and alcohol before and
Just wondering if anyone using Sakura film coverslippers has noticed streaks
along the edges of the slides. I am sure they are caused by the adhesive
leaking out and being wiped down the slide when we wipe the xylene off, but I
am wondering if anyone has any suggestions for eliminating the
Hi,
I am curious about which temperatures people are tracking on their embedding
centers for CAP, and how they are tracking them. If you are tracking the temps
for the forceps and hot and cold plates, are you using the internal thermometer
or measuring the temperature another way?
Thanks.
There is a North East Laboratory Conference that is held in Portland, Maine
every year, and each year I notice a lack of speakers pertaining to the
fascinating subject of histology. I have taken it upon myself to recruit a few
histology speakers, and I am hoping to get some talks set up for
I am looking for recommendations for microbiopsy cassettes to use on the
Primera Signature printer. I have two different types (Leica LP C and Simport
Micromesh), and neither of them print as clearly as the standard Tissue Tek
cassettes.
Thanks in advance.
Elizabeth M. Cameron, HT (ASCP),
Just wanted to add that I have used Tissue Tek Mesh biopsy cassettes in the
past and experienced issues with processing. I would also be interested in
hearing from people who use the Mesh either successfully or unsuccessfully to
determine whether that is an option.
Thanks again.
From: Cameron
Happy New Year, Histonetters!
I have always been taught that a Wright-Giemsa stain was strictly for smears,
and a different modification (i.e. May-Grunwald) should be used for tissues. I
was recently asked what the reasoning was, and to be honest I am not sure. I
understand that tissue
to adjust times.
Thanks.
From: Boyd, Debbie M [dkb...@chs.net]
Sent: Wednesday, April 16, 2014 10:17 AM
To: Cameron, Elizabeth; histonet@lists.utsouthwestern.edu
Subject: RE: Paraffin
We use Richard Allen Type 6 paraffin for both infiltration and embedding (16
Hello histonetters,
I recently made the move back to a hospital lab after being in research, and we
are currently using two different parrafins for infiltration and embedding. I
would like to change the type of paraffin that we are using (I have one in
mind), but I was wondering if there were
