[Histonet] Myophosphorylase

2014-08-15 Thread Celebre Julia
Hello Histoland, This is a two part question for all those who do muscles. 1.Is anyone having problems ordering glucose-1-phosphate? 2.Does anyone have a method for myophosphorylase that doesn't require glucose-1-phosphate? Julia Celebre Sr MLT Anatomic Pathology Hamilton Civic H

[Histonet] PFK's on muscle biopsies

2014-04-04 Thread Celebre Julia
Happy Friday !! For years now our PFK staining has been inconsistent and I'm hoping someone wouldn't mind sharing their no fail PFK method so I can make my neuropath's happy again.?? Julia Celebre Sr MLT Anatomic Pathology Hamilton Civic Hospitals 905-527-4322 ext 46179 This information is

RE: [Histonet] Isopentane storage-the polling is open

2013-04-16 Thread Celebre Julia
We store our open bottle in an explosion proof fridge and unopened at room temp in a flammable storage cabinet Julia Celebre Sr MLT Anatomic Pathology Hamilton General Hospital 905-527-4322 ext 46179 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet

[Histonet] SDH methods for muscles

2013-01-25 Thread Celebre Julia
I give up!! One week it works, the next week it doesn't, same reagents, same method, same tech, for the life of me I can't figure out what is the problem. If anybody has a fail safe method they are willing to share, please send it along. I'm pretty sure my neuropathologist will appreciate all the

[Histonet] ATP 10's for muscle biopsies

2011-10-06 Thread Celebre Julia
I'm having issues with the ATP 10's we run on our muscle biopsies and am hoping those who are familiar with this stain are willing to share their methods?! Any help or advise is greatly appreciated. Thanks Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-4322 ext 46179

[Histonet] Training first time cutters

2009-02-23 Thread Celebre Julia
> > > It's been awhile since we've had to train someone to cut from scratch and I > need your help to figure out two things: > 1. how long should it take a new cutter to cut 20 blocks, no levels, nothing > fancy, just one H&E per block. > 2. what's the average length of training for the abo

[Histonet] Flame substitute for embedding

2008-12-03 Thread Celebre Julia
Hello Histoland!! We have just been told we are no longer allowed to use alcohol burners while embedding and we are to find a safer substitute, something to do with no liking an open flame in the lab. Other than incinerators or 6 pairs of forceps at each centre, we've run out of ideas so we a