Dear All,
Please can I ask what everyone uses as a standard Eosin recipe? The new
pathologist in our department has asked for the stain to be darker, 'like the
hospital does it'. I work on the research side of a vet school, so I'm not sure
what histology departments in hospitals normally use.
I've used
fresh alcohols and solvents, clean glassware - everything I can think
of.
Can anyone give me any more ideas or possible causes? because i'm
stumped L
Thanks
Ceri Allen
Research Technician
School of Veterinary Medicine and Science
Sutton Bonnington Campus
Un
Dear Histonetters,
Does anyone know whether it is possible to remove erythrocytes from
fixed tissue?
Background: I am using an IgM antibody with a FITC conjugated secondary
on 5 micron sections of formalin fixed tissue. I'm getting a lot of
non-specific labelling of erythrocytes, but I'm n
