[Histonet] IHC

2024-03-03 Thread Chakib Boussahmain via Histonet
Greetings Histonet community, I'm interested in learning if anyone has experience withperforming Von Willebrand factor and alpha smooth muscle actin IHC staining onpig tissues (FFPE). If you do, could you please share the protocol along withdetails about the antibodies used, including

[Histonet] Bone samples

2024-01-24 Thread Chakib Boussahmain via Histonet
Hi guys,  I hope this messagefinds you well. I am currently working on a study involving bone samples thathave been treated with slow decal and embedded in paraffin. I am facingchallenges in obtaining nice sections, and I was wondering if you could providesome guidance or recommendations.

[Histonet] Tissue Quality

2021-10-05 Thread Chakib Boussahmain via Histonet
Hi Guys, We normally buy human tissue blocks to use them as controlsor to develop IF assays in our laboratory. However, most of these blocksappeared to be fixed or processed adequately. I am wondering if anybody has amethod to assess the quality of these tissue blocks besides H stain.

[Histonet] Harmony image analysis software

2020-11-18 Thread Chakib Boussahmain via Histonet
Hi Guys,I am just wondering if anyone is using Harmony image analysis software? or Opera phenix machine?I will appreciate any inputThank you in advanceChakibHistotechnologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Protocol or SOP of how to make Tissue microarray

2020-08-12 Thread Chakib Boussahmain via Histonet
Hi Histonetters,Does anyone have a written SOP or a protocol of how to make Tissue microarray that can share with me. Any input will be greatly appreciated.Thank you in advanceChakib BoussahmainHistotechnologist ___ Histonet mailing list

[Histonet] Human Tissue Blocks

2020-07-27 Thread Chakib Boussahmain via Histonet
Dear histoneters,I am working on some immunofluorescence multiplex staining/ optimization of the different kind of antibodies and I am wondering if someone can help me find human tissue block suppliers. I will need different type of tissue normal/disease and different organs.Any advice/ help