[Histonet] Control block for CDV

Hello, Would somebody happen to have an FFPE control block for CDV that we could have or barter. Thanks Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895

[Histonet] Leica Bond for TSE tesing

Hello, I am searching for veterinary labs who are using the Leica Bond to perform TSE testing following the NVSL protocol. Specifically did you or are you still having issues passing the check test? Thanks Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North

[Histonet] Bovine Corona Virus antibody

Hello, For all the Veterinary Diagnostic people. Does anybody use the BCV (BC 6-4) antibody from RTI LLC in Brocklings South Dakota?Also does anyone use the Z3A5 from Kansas State University? We have compared the two and are getting conflicting results. I do not know if the Z3A5 is

[Histonet] BVDV antibody

Hello, To all the veterinary labs. Has anyone found an antibody for BVD persistent infection by IHC on ear punch cases. The 15.c.5 clone from IDEXX is no longer available for purchase outside their ELISA test kits. I have a small amount but am going to have change soon. Thanks for all your

[Histonet] slider printer

Hello, We are looking into barcoding in our histology lab. Does anyone have recommendations on which slide printer and cassette printer works best? Pros and cons? Thanks Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110

[Histonet] eye lens

Hello, I have a block of dog eye lens in which I can get very nice sections. When I lay it out on my waterbath it wrinkles back up. Is there something I can do to keep this from happening or is there another method of mounting eye lens to a slide that someone can share. Thanks Roger Roger

[Histonet] ShurMount coverslipper

Hello, Does anyone have experience with the ShurMark glass coverslipper. We are looking at purchasing one and I would like to hear pros and cons. Thanks Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808

RE: [Histonet] Control Block Tracking

I also like to use our LIMS system as a tracking tool. In house controls have an accession number that will be on all control blocks and any out of house controls I get are accessioned into LIMS thus giving me electronic records and a case number to use on the control block. Traceability is

[Histonet] Tissue left in processor

Hi All, Is there any standard on how long tissue cassettes can remain in the processor after processing before the tissue is subjected to unwanted outcomes? And if so what type of artifacts can one expect from tissue that was in the processor in molten paraffin for 2 hours after the

[Histonet] un-nucleated squamous epithelial cells

Hello, Has anyone studied where un-nucleated squamous epithelial cells originate from? I'm leaning towards thumbing blocks with an ungloved hand but don't know for sure. Other possibilities I've come up with are dandruff from the head or uncovered arms. I hate to see our histologist work in

[Histonet] Paraffin in processor

Greetings all, We ran into an issue with the level of our first paraffin being extremely low one day. We normally rotate paraffin at 300 blocks but sometimes this could take 4-5 weeks and 15-20 runs before we get to this magic number. My question is if we change to a number of runs to

[Histonet] IHC kits

Happy Friday to all, Dako has once again raised their prices to the point that I'm asked to find another IHC labeling kit that is cheaper. Could those doing IHC please recommend a kit that is as good as Dako's LSAB2? Thanks so much. Roger Roger Charles Microbiologist II PA Veterinary

[Histonet] Laryngotracheitis

Hello, Is there someone in the veterinary field doing IHC or FA (ffpe or fresh tissue) for infectious laryngotracheitis? I'm searching for a protocol and an antibody. Thanks so much Roger Roger Charles Microbiologist II PA Veterinary Laboratory 717-787-8808

[Histonet] Processor malfunction

Happy Friday to all, Our processor shut off last evening in 80% alcohol due to a misconnection of the next reagent. We restarted this morning when we arrived but the tissues were sitting in 80% for 9 hours. My question is what affect will this have on the tissues which are a mixture of medulla

[Histonet] RE: ground meat samples

Sorry Histonetter's But it is my Friday and wouldn't this method give new meaning to the term sausage block. :) I wish Margaret all the luck in her quest and if it was me, I would try either or both methods. Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St

[Histonet] TBS vs PBS

Hello all, Would proteinase K diluted in PBS be any different then PK diluted in Tris buffer when used for a fluorescent antibody test? I'm having trouble duplicating a published testing method and the only difference is PK diluted in TBS instead of PBS. Thanks in advance for all the wonderful

[Histonet] IHC automated buffers

Hello, Is anyone presently making their own TBS with tween 20 buffer to be used in automated IHC? I would like to know if there are any problems related to these buffers and if any deviations must be made to accommodate the automation. Thanks Roger Roger Charles Microbiologist Pennsylvania

[Histonet] HE help

Hello All, TGIF I'm trying to help out one of my veterinarian pathologist in getting some information on how to increase the bluing of the HE staining. Presently we are using pre made Mayers Hematoxylin from Sigma with an automated schedule as follows: 1. Drying 45min 2. citrisolve 3

[Histonet] Paraffin wax, Thank You

Thank you to all who responded to this subject. Your knowledge will help us make our decisions on the type of paraplast to be used in our lab, at least until our contract vendor changes again. Happy Holidays to all Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N

[Histonet] Paraffin wax

Hello, Could someone share their knowledge of the Paraplast line of paraffin with me? We are being forced to change from the TissuePrep line. Thanks Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808

[Histonet] RE: Is it Formalin-Without Smelling It?

We are using Shiffs reagent. When you put a drop of formalin in Shiffs reagent it turns purple. Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On