[Histonet] Fwd: Dissolving precipitate in formalin?

654 E-mail: cmcon...@usgs.gov -- Forwarded message -- From: Lauren Sweeney Date: Tue, Aug 22, 2017 at 8:02 AM Subject: RE: [Histonet] Dissolving precipitate in formalin? To: "Conway, Carla" I've always been told that that is the salts flaking out and that you can

[Histonet] Dissolving precipitate in formalin?

Hello, I have 4 bottles of formalin which contain white flakes of paraformaldehyde precipitate. I tried to dissolve the flakes by heating to ~65 degrees C for 45 min (with stirring). Has anyone been successful in dissolving this type of precipitate? Thanks very much, Carla Carla Conway Histolog

[Histonet] Differentiating mycobacteria from Nocardia

Hello, Could anyone provide a staining protocol that will differentiate mycobacteria from Nocardia in tissue sections? The bacteria were both acid-fast-positive by the Ziehl-Neelsen stain. Thank you! Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65

[Histonet] Automatic H & E slide stainer recommendations

Hello colleagues, I would appreciate any recommendations for an automatic slide stainer. It will primarily be used for H & E staining, not IHC. Thanks in advance, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 U

[Histonet] Can acetic acid in fixative affect fungal staining by PAS?

Hello, I fixed skin/muscle and gill tissues for 48 h in Dietrich's fixative (30 mL 95 % ethanol, 10 mL formalin, 2 mL glacial acetic acid and 58 mL deionized water) and used the Hotchkiss-McManus PAS protocol to stain *Saprolegnia *fungi. The fungi are light green from the counterstain and not PAS

[Histonet] Processor malfunction - tissues not submerged ~ 9 hours

Hello everyone, When it is working correctly, our tissue processor moves a basket of tissues through 10 reagent containers. Last night it malfunctioned and suspended the tissues above the 80% ethanol container. The tissues were high and dry for ~ 9 hours (!) until I placed them into 70% ethanol th

[Histonet] Leica TP1020 tissue processor comments?

Hello, Our lab is considering purchasing a new tissue processor. We have an IMS LX-120 benchtop processor and would like another benchtop model, if possible. I'd appreciate any comments you may have regarding the Leica TP1020 processor. Thanks very much! Carla **Please note new phone number**

Re: [Histonet] Re: Fixed tissue storage ratio in 70% ethanol

Thank you, Bob! Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmcon...@usgs.gov On Fri, Jan 3, 2014 at 11:06 AM, Bob Richmond wrote: > Carla Conway at the Western

[Histonet] Fixed tissue storage ratio in 70% ethanol

Hello, When transferring formalin-fixed tissues to 70% ethanol for storage, is there a recommended ratio of ethanol volume to tissue volume? Thanks very much, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Pho

[Histonet] Xylene substitutes for tissue processor clean cycle

Hello all, The technician who does preventive maintenance on our LX-120 tissue processor recommended that we use xylene as the solvent in our clean cycle. We are considering using a xylene substitue in the clean cycle and would appreciate any recommendations. Thanks very much, Carla Conway Hist