654
E-mail: cmcon...@usgs.gov
-- Forwarded message --
From: Lauren Sweeney
Date: Tue, Aug 22, 2017 at 8:02 AM
Subject: RE: [Histonet] Dissolving precipitate in formalin?
To: "Conway, Carla"
I've always been told that that is the salts flaking out and that you can
Hello,
I have 4 bottles of formalin which contain white flakes of paraformaldehyde
precipitate. I tried to dissolve the flakes by heating to ~65 degrees C for
45 min (with stirring). Has anyone been successful in dissolving this type
of precipitate?
Thanks very much,
Carla
Carla Conway
Histolog
Hello,
Could anyone provide a staining protocol that will differentiate
mycobacteria from Nocardia in tissue sections? The bacteria were both
acid-fast-positive by the Ziehl-Neelsen stain.
Thank you!
Carla
Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65
Hello colleagues,
I would appreciate any recommendations for an automatic slide stainer. It
will primarily be used for H & E staining, not IHC.
Thanks in advance,
Carla
Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 U
Hello,
I fixed skin/muscle and gill tissues for 48 h in Dietrich's fixative (30 mL
95 % ethanol, 10 mL formalin, 2 mL glacial acetic acid and 58 mL deionized
water) and used the Hotchkiss-McManus PAS protocol to stain
*Saprolegnia *fungi.
The fungi are light green from the counterstain and not PAS
Hello everyone,
When it is working correctly, our tissue processor moves a basket of
tissues through 10 reagent containers. Last night it malfunctioned and
suspended the tissues above the 80% ethanol container. The tissues were
high and dry for ~ 9 hours (!) until I placed them into 70% ethanol th
Hello,
Our lab is considering purchasing a new tissue processor. We have an IMS
LX-120 benchtop processor and would like another benchtop model, if
possible. I'd appreciate any comments you may have regarding the Leica
TP1020 processor.
Thanks very much!
Carla
**Please note new phone number**
Thank you, Bob!
Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-6282 ext. 242
Fax: 206-526-6654
E-mail: cmcon...@usgs.gov
On Fri, Jan 3, 2014 at 11:06 AM, Bob Richmond wrote:
> Carla Conway at the Western
Hello,
When transferring formalin-fixed tissues to 70% ethanol for storage, is
there a recommended ratio of ethanol volume to tissue volume?
Thanks very much,
Carla
Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Pho
Hello all,
The technician who does preventive maintenance on our LX-120 tissue
processor recommended that we use xylene as the solvent in our clean cycle.
We are considering using a xylene substitue in the clean cycle and would
appreciate any recommendations.
Thanks very much,
Carla Conway
Hist
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