Re: [Histonet] Paraplast

2010-06-07 Thread DELIA GARCIA
We use Richard Allan Paraffin type 3 for infiltration and type 6 for embedding. We cut at 3 mircrons and we ribbon out real nice! From: Rebecca Johnson r...@bluemarble.net To: histonet Histonet@lists.utsouthwestern.edu Sent: Thu, June 3, 2010 1:20:37 PM

Re: [Histonet] ?New CAP guidelines affect CLIA '88

2010-04-27 Thread DELIA GARCIA
I too am curious about this issue. Any info would be oh so greatly appreciated.   Thanks --- On Tue, 4/27/10, Barone, Carol cbar...@nemours.org wrote: From: Barone, Carol cbar...@nemours.org Subject: [Histonet] ?New CAP guidelines affect CLIA '88 To: histonet@lists.utsouthwestern.edu Date:

[Histonet] Need CLIA help

2010-04-14 Thread DELIA GARCIA
Hello Histonetters,   There are two issues that have been boggling me that I really cant find a definite answer for. I am hoping to learn from your experiences and what works. Anything helps.   I work in a CLIA regulated Derm Lab. I would like to ask what is CLIA's requirement at the grossing

[Histonet] Nail softner

2009-12-31 Thread DELIA GARCIA
Hi Everyone,   I have a question... What is a good nail softner to use at the grossing table prior to fixation? We were currently using KOHw/DMSO from Healthlink Healthcare. However I cannot find who sells it. Healthlink only distributes. Plus I dont have the time to keep hunting for it. I'm

[Histonet] Cryostat

2009-12-31 Thread DELIA GARCIA
  We are a Derm Lab and we use ours quite a bit. We do a manual defrost monthly. We clean it daily. It is also set to auto defrost nightly.     Delia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] arizona state license

2009-12-09 Thread DELIA GARCIA
No we dont. At least not yet. However certification is strongly preferred around here. -Original Message- Date: Wednesday, December 09, 2009 4:39:04 pm To: 'histonet' Histonet@lists.utsouthwestern.edu From: dcoj...@tampabay.rr.com Subject: [Histonet] arizona state license Does anyone

[Histonet] Thanks!!! Re: hematoxylin

2009-11-12 Thread DELIA GARCIA
Thank you all for your responses. I have tried a few things. But still going at it. :-) Have a great day! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Processing small gi biopsies, skin lesions, currettings

2009-11-05 Thread DELIA GARCIA
Thanks for throwing this one out there. I am also interested in this topic. Can you please post replies to the net? Thanks !!! :-) --- On Thu, 11/5/09, Vickroy, Jim vickroy@mhsil.com wrote: From: Vickroy, Jim vickroy@mhsil.com Subject: [Histonet] Processing small gi biopsies, skin

RE: [Histonet] Transporting Formalin

2009-10-13 Thread DELIA GARCIA
We have various shipping addresses with our supplier. Which allows us to order directly for our clients. We incur all costs including the associated Hazardous Materials handling fee. We also use courier for in-town clients. Hope this helps. Delia Garcia Lead Histotechnologist Tucson, AZ

[Histonet] Mouse Vs. Rabbit

2009-08-09 Thread DELIA GARCIA
Hi, I am  new to IHC so please excuse me if I dont ask this question properly. What is the difference between a mouse clone and rabbit clone? I came across a decision I had to make while purchasing an antibody. It was mentioned that usually a rabbit clone is the preferred. Is that true and

[Histonet] Please Help!

2009-07-31 Thread DELIA GARCIA
Hello, I am in dire need of some suggestions. I work for a Derm lab and we occasionally get fresh skin punches that we freeze to do FITC (immunoflouresence). I have a really hard tiime keeping the tissue from curling makeing almost impossible to get a decent section on the slide. Anyone out

[Histonet] from PLEASE HELP

2009-07-31 Thread DELIA GARCIA
Thank you all for your imput. I tried thinning out the sections to 3 mircrons and for some reason cut a bit better. There's a few more things you all mentioned Im going to try. The thing with the punch biopsies is that when when i'm cutting the frozen the actual tissue is what curls leaving a