[Histonet] unsubscribe

I would like to unsubscribe to this list Regards Daniela Bodemer Research Assistant Surgical Research, Infection & Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia E daniela.bode...@mcri.edu.au<mailto:

[Histonet] Fixation time

Hi all, Our tissue processor has been shut down due to a contamination issue and now all the tissues (mice and rat pelves) collected prior to this happening have been sitting in 4% PFA. Some tissues more than a week, when we usually fix for 48 hours. Now we are transferring the tissues to 70%

[Histonet] Frozens and antigen retrieval

Hi all, A question from my student: antigen retrieval when using frozens for immunofluorescence -yes or no? The protocol suggested by the antibody company lists the retrieval as an option. I am used to do retrieval on paraffin sections, but not on cryo sections. Hit me with your opinions on

[Histonet] Macrophage stain

Hi all, I would like to stain for macrophages in mice aorta (5um frozen sections). Would you have suggestions on which stains to use and perhaps share the protocol? Thanks in advance, Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens

[Histonet] FW: Oil Red O

From: Daniela Bodemer Sent: Thursday, 16 February 2012 9:59 AM To: Histonet@lists.utsouthwestern.edu Subject: Oil Red O Hi all, I am staining cryo sections of aorta for lipids with Oil Red O and mounting my slides with Mowiol. My question is how long will they last without fading

[Histonet] Oil Red O

Hi all, I am staining cryo sections of aorta for lipids with Oil Red O and mounting my slides with Mowiol. My question is how long will they last without fading and should they be kept in a folder in the fridge? Thanks for your thoughts, Daniela Daniela Bodemer Research

RE: [Histonet] Immunofluorescence

Hi Natalia, Human tissue is naturally autofluorescent. In our lab we do immunos on frozens and quench the AF with chicago blue solution (1g Chicago Sky Blue 6B-Sigma in 99ml MilliQ plus 1ml DMSO) after washing the secondary antibodies with PBS (3x). Just load the tissue with a drop of the CB solut

[Histonet] Quenching auto fluorescence in human tissue

use Mowiol as a mounting medium. Is the mounting medium reacting with Chicago Blue? Is there any alternative to quench die auto fluorescence of the tissue? Thanks for your ideas. Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research

[Histonet] gelatine, silane, super frost plus slides ???

Hi all, I am putting information together for students and thought you might be able to help me. Gelatine, silane, super frost plus slides. What are the differences and characteristics and which slide to use for what? Thank you, Daniela Bodemer

FW: [Histonet] Chicago Sky Blue stain vs. KOH - striking comparison

We use Chicago Sky Blue 6B from Sigma C-8679 (2610-05-1). We make our solution as followed: 1g CSB 6B 1ml DiMethylsulphoxid 99ml Dist water Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hos

[Histonet] DAB Immuno and alcian blue counter staining

Hi all, Is it possible to do an immuno with DAB detection and counterstain it with alcian blue at the end to detect Goblets cells? I've tried it and the DAB disappears under the staining. Thanks, Daniela __ This email has been

RE: [Histonet] Problems with Frozen tissues

Our tissues are frozen in OCT. I use Super Frost Plus slides from Thermo Scientific and store the sections in the fridge until I need them. After leaving them out on the bench for an hour I start my H&E with 1 min running water and so on. I rarely lose sections with these slides. Daniela -

RE: [Histonet] processor stopped - help!

I am glad to hear the samples will be fine. Thanks everyone! Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9345 5930

[Histonet] processor stopped - help!

or what can I do to save the samples? Thanks for your advice, Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9345 5930

[Histonet] Reorienting frozen tissue

Hi all, I would like to know if it is possible and how to reorient or reembedd human tissue, that has been frozen at -80C in OCT. Many thanks, Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children&#