Thanks, Liz. We have not tried that for PSR before but could easily be tested.
Thanks for sending the protocol!
David
From: Liz Chlipala
Sent: Thursday, June 22, 2023 9:36 AM
To: David Burk ; John Kiernan ;
histonet@lists.utsouthwestern.edu
Subject: RE: Fast
From: John Kiernan
Sent: Wednesday, June 21, 2023 11:36 PM
To: histonet@lists.utsouthwestern.edu ;
David Burk
Subject: Re: Fast Green / Sirius Red - Unknown blue features
Your technique is the one first (I think) published by Lopez-De Leon A &
Rojkind M (1985) A simple micromethod for coll
=sharing
Mouse skeletal muscle:
https://drive.google.com/file/d/10vT_FKu3-Ad5uemM5gnZmqDCKb3fs2lV/view?usp=sharing
Thanks,
David Burk
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I just wanted to say Thanks! to all those who responded to my question
on ORO staining formalin fixed cryoprotected mouse tissue. The general
consensus was that it was perfectly fine to do so with the caveat that
sometimes sections don't want to stick to slides.
Thanks again!
David
Experts!
Is there any reason you would not consider formalin fixation followed by
cryoprotection/cryosectioning for Oil Red O (or bodipy for that matter)
staining of mouse muscle?
I ask as many folks seem to have difficulty in the flash freezing aspect
of tissue collection and end up with
in to using xylene-free processing methodology if at all
possible but will entertain any suggestions you may have.
If I can provide any further details about what we are doing on our
end, please let me know and I'll be happy to provide them.
Best,
David Burk
xylene-free processing methodology if at all possible
but will entertain any suggestions you may have.
If I can provide any further details about what we are doing on our end,
please let me know and I'll be happy to provide them.
Best,
David Burk
