Hi Histonet, Marcus and Liz
I agree with everything Liz says about precipitated DAB being wonderfully
robust so that you can do a whole range of stains successfully after using it.
I am slightly puzzled that Marcus wants to reveal RBCs after a DAB reaction.
Doesn't it just happen any way? Haem/
Hi Histonet & Maria
Is it possible that Maria really means Nissl staining of neuronal cell bodies
(somata) together with their nuclei, rather than just nuclei? Tim is right to
point out that the staining target is likely getting washed away during IHC
processing, but I suggest it is Nissl subst
Hi Yves & Histonet
It is certainly a good sign if limbs etc are stiff after perfusion, but maybe
not a guarantee that the target organ is perfect given the short perfusions you
describe. Definitely, if I don't see stiffness I worry, check for a torn aortic
arch (you are doing it transcardially,
