Re: [Histonet] her 2 controls

2019-04-18 Thread Dessoye, Michael via Histonet
Check out Histocyte controls, which are available on slides or blocks. One of their distributors is Cell Marque: http://www.cellmarque.com/cms/histocyte/HER2.php Their Her2 control include 0, 1+, 2+, and 3+. Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor |  Common

[Histonet] ER/PR/Her2 control

2019-04-08 Thread Dessoye, Michael via Histonet
Hello Histonet! Looking for some advice regarding ER/PR/Her2 controls. I'm able to buy commercially TMA blocks containing varying levels of positivity for ER, PR, and Her2. This work nicely because you can have a negative and varying levels of positive control as a same-slide control. But ou

[Histonet] ER/PR/Her2 Controls

2019-03-20 Thread Dessoye, Michael via Histonet
Hello Histonet, Can anyone recommend a commercial source of ER/PR/Her2 control tissue (array blocks would be preferred)? I'm looking for something similar to the breast analyte control from Cell Marque, which is RUO: http://www.cellmarque.com/cms/histocyte/breast-DR.php Thanks, Mike Michael

Re: [Histonet] glass slides

2019-01-29 Thread Dessoye, Michael via Histonet
That's interesting, we've been seeing warping with the Leica coverslips. I wonder if there's something in common in their manufacturing. We've been using a mixture of Superfrost Plus (Fisher), Basix (Fisher), and Platinum line (Mercedes Medical) all with good results. The 'Basix' seems to be

[Histonet] Specimen disposal bags

2019-01-04 Thread Dessoye, Michael via Histonet
Hello Histonet, I'm looking for some recommendations on biohazard bags for specimen disposal. Currently using Brighton 3.0 mil 44 gallon bags and they have occasional problems with leakage. Obviously the formalin is removed but there is always some residual formalin present. I'm willing to p

[Histonet] TMA slides for IHC validation

2018-10-15 Thread Dessoye, Michael via Histonet
I'm using TMA slides from US Biomax to assist with IHC validation. I'm working on p120 on a Benchmark Ultra and BR2410 slides from Biomax. The cases are all invasive lobular carcinoma, which should all stain positively, but only about half are staining positive. I sent an identical slide to o

Re: [Histonet] IHC validation

2018-03-22 Thread Dessoye, Michael via Histonet
Can anyone recommend a vendor that they've had good luck with for TMA slides? Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor |  Commonwealth Health Laboratory Services | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-14

Re: [Histonet] ClearRite

2017-06-12 Thread Dessoye, Michael via Histonet
We've used ClearRite 3 and had no issues with IHC or molecular. Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor |  Commonwealth Health Laboratory Services | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570

Re: [Histonet] P16

2016-06-08 Thread Dessoye, Michael via Histonet
Ventana/Roche bought out MTM which had the patent, so they're the only vendor you can get it from. Since you're using Bond you obviously don't want it in a dispenser, but you can buy their manual staining kit and just use the antibody from that. Michael J. Dessoye, M.S. | Histology/Toxicology/

[Histonet] Air filter/purifier

2016-05-04 Thread Dessoye, Michael via Histonet
Hello Histonet, Does anyone have experience with an air filter/purifier for use in the lab? I'm looking mainly for residual toluene fumes. Everything is always below the limit but I have an employee who is unusually sensitive and I'm trying to help out. I found one from Fisher for an astrono

[Histonet] Peloris baskets

2016-03-09 Thread Dessoye, Michael via Histonet
Hello Histonet, Does anyone know of any baskets (preferably spaced) that are compatible with the Leica Peloris? I'm looking for alternatives to the astronomically-priced factory baskets. Any thoughts? Thanks, Mike Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre

Re: [Histonet] Price gun

2016-01-08 Thread Dessoye, Michael via Histonet
Check this out: http://www.staples.com/Garvey-reg-1-Line-Pricemarker/product_69516 Lots of types available at Staples, Grainger, etc. It will save you a lot of writing. Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealt

[Histonet] Cryostat vacuum

2015-09-09 Thread Dessoye, Michael via Histonet
Hello Histonet, Does anyone know where I can find a cryostat vacuum? I've used them in the past but can't seem to find a manufacturer. Thanks! Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@comm