[Histonet] cleaning fluid & lubricating fluid discontinued

Histonetters, Fisher-Scientific has discontinued selling Microsharp Cleaning Fluid and Microsharp Lubricating Fluid that I use for sharpening stainless steel knives on my Shandon Autosharp 5 knife sharpener. Can anyone recommend a comparable substitute that I can use in their place? Vendor's

RE: [Histonet] Protocol for decalcifying and processing large bone section.

I use 10% formic acid for large human bones but I don't think your bone sections are that large. Peggy DiCarlo Ortho Bone Lab BGMC 100 High St. Buffalo, NY 14203 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

[Histonet] question about refrigeration

Histonetters, I was wondering how long a bone specimen (portion of pt's tibia in this case) can be in a refrigerator before it will decompose and not produce a good quality section? The case was completed at 3:30 pm, refrigerated and then put into formalin at 8:15 am the next day? I am

[Histonet] 35 mm scanners

Everyone, I currently use a Nikon Digital Ice 3, Coolscan IV ED to scan 35 mm photos. Lately, it is often scanning a magenta color or at times either a faded or blurry image and I know I will need to replace it soon. Can anyone recommend a reasonably priced, good quality 35 scanner? It

RE: [Histonet] difficult cross section to cut

Of DiCarlo, Margaret Sent: Tuesday, January 10, 2012 12:35 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] difficult cross section to cut Histonetters, I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a femur that they think is osteoporotic. After decaling the cross

[Histonet] difficult cross section to cut

Histonetters, I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a femur that they think is osteoporotic. After decaling the cross section of the femur in 10% formic acid for 12 days, processing using xylene and embedding in Tissue Prep 2, I have been unsuccessful in attaining

[Histonet] aluminum gutter guard

Hello Histonetters, I have been using aluminum gutter guard to wrap my bones when I manually process them- from increasing percentages of alcohols to xylene and paraffin. I am running very low on it and I have not been able to find it at any hardware stores. I looked on line and Amazon is out

[Histonet] bone-freezing versus formalin fixing

Hello, I work for two orthopedic surgeons and one would like his whole bone frozen at -80 degrees Celsius and the other one would like it fixed in 10% buffered formalin before slicing on a band saw. I have to photograph and x-ray upon receiving them which isn't always the same day of surgery.

[Histonet] different microtome?

Hello Everyone, I am attempting to cut decaled (with EDTA) dog bone tissue which is attached to a partial tooth, manually processed and embedded in Tissue Prep 2 paraffin. I have cut one block but have only gotten thick and thin sections. I am currently using a Microm HM 325 using high

RE: [Histonet] bottom of water bath

responses. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 -Original Message- From: Pamela Marcum [mailto:[EMAIL PROTECTED] Sent: Thursday, September 11, 2008 16:25 To: 'Geoff McAuliffe'; DiCarlo, Margaret Cc: [EMAIL PROTECTED] Subject