Was wondering if anyone would be willing to share their protocols for the Ventana Kappa and Lambda ASR probes for the Benchmark XT. I have been having a time trying to get these protocols worked out. When I ran the probes the first couple of times with the same protocol as the old probes, they were beautiful. The probes then stopped working all together with no staining at all. I adjusted the stringency wash temp and now I have a ton of background staining. Any suggestions would be greatly appreicated.
Thanks, Holly L. Dorsey, HT CMC-NE, Concord NC ________________________________ This electronic message may contain information that is confidential and/or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
