Hello all,
I use microwave processing exclusively. First of all, are you making sure
that each reagent is completely extracted before the next reagent is
administered? I know the tissue cannot be completely dry, but all residue
reagent should be siphoned off before the next one goes in. Thi
Hi Brian,
I regularly use epon for TEM work. Even though the instructions are good for
some climates , epon is particular.
Here in Houston, I let my blocks cure for 5 to 7 days depending on how humid or
rainy it is. When I press a fingernail into the block , if it leaves a mark I
know it need
Congratulations Tim,
I too was a graduate of the San Joaquin Delta EM School under Dr Murphy. Your
story is similar to most of us. We have been able to go through doors that
others only wish they could. Over the years , we often experience many
accomplishments, and get to meet many colleague
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I agree with Rene, and everyone else that using random generated tracking
numbers are a really, really, big mistake. We do EM here and it would be a
nightmare if we used random numbers. There are ways to use tracking numbers
where the ID of an individual is not compromised. Perhaps NIH should
Hello Histoland,
Someone needs help in our lab with some fluorescent staining. He is staining
drosophila retina with antibody PI4.5P2, or PIP2 for looking at Phospholipids
around the membranes. He is using 4% Paraformaldehyde and Triton X in his
protocol and is getting inconsistent results
Hi Allyse,
TEM is a whole new ball of wax so to speak. It depends on what you are fixing,
and how you plan to fix it. Typically you would start with Paraformaldehyde
and a Glutaraldehyde mix such as Karnovsky's. The after water washes do a
secondary fix in Osmium Tetroxide followed by water w
Hello to all Histologists,
Perhaps someone can help me. At our institution we primarily use electron
microscopy for slides with toluidine blue, and grids for TEM. The students
here also do some immunostaining on their tissues. We have a student though
who would like to try using the Acid Pho
Philip,
Try also Diatome Diamond Knives. http://www.diatomeknives.com/
Regards,
Lita Duraine
EM Technologist
Bellen Lab
HHMI- Molecular Genetics
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mitchel
Hello Histonet Users,
I have decided that I should ask the experts. I regularly thick section muscle
sections in Embed 812 for bouton identification. I normally use Toluidine
blue. I am having trouble differentiating between boutons and a type of
vacuole that occurs within the same tissue and
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