RE: [Histonet] GemCut Colored Paraffin

2009-04-02 Thread Emily Lockman
We use this paraffin and it does not cause any permanent color change to the tissue. When it is removed from the processor after infiltration, the tissue does have some color, but after deparaffinization during staining, there is no color left. Hope this helps. Emily M. Lockman, HT (ASCP) Histote

[Histonet] deplasticizing Spurr slides

2009-04-01 Thread Emily Lockman
I still need help with deplasticizing our Spurr slides. Any advice would be helpful. Also, when cutting Spurr sections, what type of slides (charged, coated, ect.) are people using? Thanks, Emily Emily M. Lockman, HT (ASCP) Histotechnologist II, Pathology Services American Preclinic

[Histonet] deplastisizing Spurr

2009-03-23 Thread Emily Lockman
I'm wondering if anyone has any helpful tips for deplastisizing Spurr. We currently use saturated Sodium Hydroxide in 100% alcohol. We tried a 5% and a 1% solution as well, but still not perfect. The main issue we are having is the tissue is lifting off of the slide during staining. For microtomy